Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Human

Predicted:

Rat (100%). Backed by our 100% Guarantee.

Applications

Immunoprecipitation (Negative), Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

1.0 mg/ml

View all MID1 Antibodies »

Product Specifications

Immunogen

The immunogen recognized by this antibody maps to a region between residue 617 and 667 of human midline 1 using the numbering given in entry NP_000372.1 (GeneID 4281).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for MID1 Antibody

Western Blot: MID1 Antibody [NBP1-26612]

Western Blot: MID1 Antibody [NBP1-26612]

Western Blot: MID1 Antibody [NBP1-26612] - Whole cell lysate from HeLa (5, 15 and 50 ug) and 293T (T; 50 ug) cells. Antibody used at 1 ug/ml.

Applications for MID1 Antibody

Application
Recommended Usage

Western Blot

1:2000-1:10000
Application Notes
Not recommended for immunoprecipitation. Western blot of lysates performed using standard western blot reagents and 4-8% SDS-PAGE.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: MID1

Defects in the Midline-1 (MID1) gene are the cause of Opitz syndrome, an X-linked recessive disorder characterized by developmental defects. MID1 is a microtubule-associated protein (MAP) with E3 ubiquitin ligase activity. MID1 has been shown to target protein phosphatases 2A (PP2Ac) on microtubules for degradation. MID1 association with PP2A has been proposed to regulate microtubule stabilization for cellular processes such as cell division and migration.

Alternate Names

EC 6.3.2.-, FLJ57031, FLJ58683, FLJ76288, FXYGBBB1, Midin, midline 1 (Opitz/BBB syndrome), RNF59MIDIN, TRIM18mouse, homolog of

Entrez Gene IDs

4281 (Human)

Gene Symbol

MID1

UniProt

NP_000372.1 (Human)

Additional MID1 Products

Product Documents for MID1 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for MID1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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