MID1 Antibody - BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-05061
Key Product Details
Species Reactivity
Validated:
Human, Mouse
Applications
Immunocytochemistry/ Immunofluorescence, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Summary for MID1 Antibody - BSA Free
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 478-667 of human MID1 (NP_000372.1). KLKTNSQPFKLDPKSAHRKLKVSHDNLTVERDESSSKKSHTPERFTSQGSYGVAGNVFIDSGRHYWEVVISGSTWYAIGLAYKSAPKHEWIGKNSASWALCRCNNNWVVRHNSKEIPIEPAPHLRRVGILLDYDNGSIAFYDALNSIHLYTFDVAFAQPVCPTFTVWNKCLTIITGLPIPDHLDCTEQLP
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
75 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for MID1 Antibody - BSA Free
![Western Blot: MID1 AntibodyBSA Free [NBP3-05061]](https://resources.bio-techne.com/images/products/MID1-Antibody-Western-Blot-NBP3-05061-img0003.jpg "Western Blot: MID1 AntibodyBSA Free [NBP3-05061]")
Western Blot: MID1 AntibodyBSA Free [NBP3-05061]
Western Blot: MID1 Antibody [NBP3-05061] - analysis of extracts of HeLa cells, using MID1 Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit. Exposure time: 60s.![Immunocytochemistry/ Immunofluorescence: MID1 Antibody - BSA Free [NBP3-05061]](https://resources.bio-techne.com/images/products/MID1-Antibody-Immunocytochemistry-Immunofluorescence-NBP3-05061-img0001.jpg "Immunocytochemistry/ Immunofluorescence: MID1 Antibody - BSA Free [NBP3-05061]")
Immunocytochemistry/ Immunofluorescence: MID1 Antibody - BSA Free [NBP3-05061]
Immunocytochemistry/Immunofluorescence: MID1 Antibody [NBP3-05061] - Analysis of MCF-7 cells using MID1 antibody . Blue: DAPI for nuclear staining.Applications for MID1 Antibody - BSA Free
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:50-1:100
Western Blot
1:500-1:2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS with 50% glycerol, pH7.3.
Format
BSA Free
Preservative
0.02% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: MID1
Alternate Names
EC 6.3.2.-, FLJ57031, FLJ58683, FLJ76288, FXYGBBB1, Midin, midline 1 (Opitz/BBB syndrome), RNF59MIDIN, TRIM18mouse, homolog of
Gene Symbol
MID1
Additional MID1 Products
Product Documents for MID1 Antibody - BSA Free
Product Specific Notices for MID1 Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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