MID2 Antibody - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-05060

Novus Biologicals, part of Bio-Techne
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NBP3-05060-100ul
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Key Product Details

Species Reactivity

Validated:

Human, Mouse

Applications

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all MID2 Antibodies »

Product Summary for MID2 Antibody - BSA Free

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 476-705 of human MID2 (NP_438112.2). GTRYIFIVKAINQAGSRNSEPTRLKTNSQPFKLDPKMTHKKLKISNDGLQMEKDESSLKKSHTPERFSGTGCYGAAGNIFIDSGCHYWEVVMGSSTWYAIGIAYKSAPKNEWIGKNASSWVFSRCNSNFVVRHNNKEMLVDVPPHLKRLGVLLDYDNNMLSFYDPANSLHLHTFDVTFILPVCPTFTIWNKSLMILSGLPAPDFIDYPERQECNCRPQESPYVSGMKTCH

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for MID2 Antibody - BSA Free

Western Blot: MID2 AntibodyBSA Free [NBP3-05060]

Western Blot: MID2 AntibodyBSA Free [NBP3-05060]

Western Blot: MID2 Antibody [NBP3-05060] - Analysis of extracts of various cell lines, using MID2 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit

Applications for MID2 Antibody - BSA Free

Application
Recommended Usage

Western Blot

1:500-1:2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS with 50% glycerol, pH7.3.

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: MID2

MID2 is a member of the tripartite motif (TRIM) family. The MID motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. The protein localizes to microtubular structures in the cytoplasm. Its function has not been identified. Alternate splicing of the gene encoding MID2 results in two transcript variants encoding different isoforms.

Alternate Names

EC 6.3.2.-, FLJ37715, FLJ41813, FXY2midin-2, midin 2, Midin-2, midline 2, Midline defect 2, Midline-2, RING finger protein 60, RNF60midline-2, TRIM1probable E3 ubiquitin-protein ligase MID2, tripartite motif protein 1, Tripartite motif-containing protein 1

Gene Symbol

MID2

Additional MID2 Products

Product Documents for MID2 Antibody - BSA Free

Product Datasheets

Certificate of Analysis

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Product Specific Notices for MID2 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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