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midA Antibody (OTI4A5)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-00820

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Human, Mouse

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # OTI4A5

Concentration

0.86 mg/ml

Product Specifications

Immunogen

Full length human recombinant protein of human C2orf56(NP_653337) produced in HEK293T cell.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Theoretical MW

49.1 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for midA Antibody (OTI4A5)

Western Blot: midA Antibody (4A5) [NBP2-00820] Analysis of extracts (35ug) from 9 different cell lines by using anti-midA monoclonal antibody.
Immunohistochemistry-Paraffin: midA Antibody (OTI4A5) [NBP2-00820]

Immunohistochemistry-Paraffin: midA Antibody (OTI4A5) [NBP2-00820]

Immunohistochemistry-Paraffin: midA Antibody (4A5) [NBP2-00820] - Staining of paraffin-embedded Human tonsil using anti-midA mouse monoclonal antibody.
Western Blot: midA Antibody (OTI4A5) [NBP2-00820]

Western Blot: midA Antibody (OTI4A5) [NBP2-00820]

Western Blot: midA Antibody (4A5) [NBP2-00820] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY midA (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-midA.

Applications for midA Antibody (OTI4A5)

Application
Recommended Usage

Immunohistochemistry

1:150

Immunohistochemistry-Paraffin

1:150

Western Blot

1:500-2000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.3), 1.0% BSA and 50% Glycerol

Preservative

0.02% Sodium Azide

Concentration

0.86 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: midA

Alternate Names

chromosome 2 open reading frame 56, MidA, mitochondrial, PRO1853

Entrez Gene IDs

55471 (Human)

Gene Symbol

NDUFAF7

Additional midA Products

Product Documents for midA Antibody (OTI4A5)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for midA Antibody (OTI4A5)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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