midA Antibody (OTI4A5)
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-00820
Conjugate
Catalog #
Forumulation
Catalog #
Key Product Details
Species Reactivity
Human, Mouse
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2B Clone # OTI4A5
Concentration
0.86 mg/ml
Product Specifications
Immunogen
Full length human recombinant protein of human C2orf56(NP_653337) produced in HEK293T cell.
Reactivity Notes
Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2B
Theoretical MW
49.1 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for midA Antibody (OTI4A5)
Western Blot: midA Antibody (4A5) [NBP2-00820] Analysis of extracts (35ug) from 9 different cell lines by using anti-midA monoclonal antibody.
Immunohistochemistry-Paraffin: midA Antibody (OTI4A5) [NBP2-00820]
Immunohistochemistry-Paraffin: midA Antibody (4A5) [NBP2-00820] - Staining of paraffin-embedded Human tonsil using anti-midA mouse monoclonal antibody.Western Blot: midA Antibody (OTI4A5) [NBP2-00820]
Western Blot: midA Antibody (4A5) [NBP2-00820] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY midA (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-midA.Applications for midA Antibody (OTI4A5)
Application
Recommended Usage
Immunohistochemistry
1:150
Immunohistochemistry-Paraffin
1:150
Western Blot
1:500-2000
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
PBS (pH 7.3), 1.0% BSA and 50% Glycerol
Preservative
0.02% Sodium Azide
Concentration
0.86 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: midA
Alternate Names
chromosome 2 open reading frame 56, MidA, mitochondrial, PRO1853
Entrez Gene IDs
55471 (Human)
Gene Symbol
NDUFAF7
Additional midA Products
Product Documents for midA Antibody (OTI4A5)
Product Specific Notices for midA Antibody (OTI4A5)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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