MIF4GD Antibody (OTI4C11)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-01876

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # OTI4C11

Concentration

0.78 mg/ml

View all MIF4GD Antibodies »

Product Specifications

Immunogen

Full length human recombinant protein of human MIF4GD(NP_065730) produced in HEK293T cell.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

29.2 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for MIF4GD Antibody (OTI4C11)

Western Blot: MIF4GD Antibody (OTI4C11) [NBP2-01876]

Western Blot: MIF4GD Antibody (OTI4C11) [NBP2-01876]

Western Blot: MIF4GD Antibody (4C11) [NBP2-01876] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MIF4GD (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MIF4GD.
Immunocytochemistry/Immunofluorescence: MIF4GD Antibody (4C11) [NBP2-01876] Staining of COS7 cells transiently transfected by pCMV6-ENTRY MIF4GD.
Flow Cytometry: MIF4GD Antibody (OTI4C11) [NBP2-01876]

Flow Cytometry: MIF4GD Antibody (OTI4C11) [NBP2-01876]

Flow Cytometry: MIF4GD Antibody (4C11) [NBP2-01876] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-MIF4GD antibody, and then analyzed by flow cytometry.
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Applications for MIF4GD Antibody (OTI4C11)

Application
Recommended Usage

Flow Cytometry

1:100

Immunocytochemistry/ Immunofluorescence

1:100

Western Blot

1:2000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.3), 1.0% BSA and 50% Glycerol

Preservative

0.02% Sodium Azide

Concentration

0.78 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: MIF4GD

This gene encodes a protein which interacts with the N-terminus of the stem-loop binding protein (SLBP) and the 3' end of histone mRNA. This interaction facilitates the activation of histone mRNA translation. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq].

Alternate Names

AD023, hSLIP1, MGC45027, MIF4G domain containing, MIFD, SLBP (stem loop binding protein)-interacting protein 1, SLBP-interacting protein 1, SLIP1MIF4G domain-containing protein

Entrez Gene IDs

57409 (Human)

Gene Symbol

MIF4GD

Additional MIF4GD Products

Product Documents for MIF4GD Antibody (OTI4C11)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for MIF4GD Antibody (OTI4C11)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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