We are working on a Sandwich ELISA platform for AMH assay. We have the following queries regarding the AMH antibodies: 1. Do you recommend any pairs of the antibodies for Sandwich Elisa platform for Human AMH protein detection? 2. Were the antibodies raised against recombinant protein or synthetic peptide or Native AMH protein? 3. What was the molecular weight of the AMH protein (140KDa or 22KDa) used to check the specificity of the antibodies?

Name: MIS/AMH Antibody
Brand: Novus Biologicals
SKU: NBP1-70639

In regards to your sandwich ELISA inquiry, I was able to find a potential pair of antibodies that may work for your experiment (catalog numbers NBP1-70639 and NBP2-11800). Neither of these antibodies has been tested in ELISA or as a pair, so we cannot guarantee them in this application. The monoclonal antibody was raised against the human recombinant AMH protein. The polyclonal was raised against an AMH peptide. Since we do not epitope map the monoclonal, we cannot say for certain that it will not bind to a similar region as the polyclonal. If you would like to test these in ELISA, then I can recommend our Innovator's Reward program. The theoretical and observed molecular weight for this protein is 59kDa. You can find more information on UniProt about this protein: UniProt P03971.

MIS/AMH Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-70639

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Product Specifications
Scientific Data
Applications
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

0.5 mg/ml

View all MIS/AMH Antibodies »

Product Specifications

Immunogen

Synthetic peptides corresponding to AMH(anti-Mullerian hormone) The peptide sequence was selected from the middle region of AMH. Peptide sequence SVDLRAERSVLIPETYQANNCQGVCGWPQSDRNPRYGNHVVLLLKMQARG. The peptide sequence for this immunogen was taken from within the described region.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

60 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

The addition of 50% glycerol is optional for those storing this antibody at -20C and not aliquoting smaller units. However, please note that glycerol may interrupt some downstream antibody applications and should be added with caution.

Scientific Data Images for MIS/AMH Antibody

Western Blot: MIS/AMH Antibody [NBP1-70639]

Western Blot: MIS/AMH Antibody [NBP1-70639] - Sample Tissue: Human Fetal Lung Antibody Dilution: 1.0 ug/ml

Immunohistochemistry: MIS/AMH Antibody [NBP1-70639]

Immunohistochemistry: MIS/AMH Antibody [NBP1-70639] - Human Prostate lysate tissue at an antibody concentration of 5.0 ug/ml using anti-AMH antibody

Western Blot: MIS/AMH Antibody [NBP1-70639]

Western Blot: MIS/AMH Antibody [NBP1-70639] - Titration: 1 ug/ml Positive Control: Human Fetal Small Intestine cell lysate.

Applications for MIS/AMH Antibody

Application

Recommended Usage

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Paraffin

1:10-1:500

Western Blot

1.0 ug/ml

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, 2% Sucrose

Preservative

0.09% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: MIS/AMH

Anti-Mullerian hormone is a member of the transforming growth factor-beta gene family which mediates male sexual differentiation. Anti-Mullerian hormone causes the regression of Mullerian ducts which would otherwise differentiate into the uterus and fallopian tubes. Some mutations in the anti-Mullerian hormone result in persistent Mullerian duct syndrome.Anti-Mullerian hormone is a member of the transforming growth factor-beta gene family which mediates male sexual differentiation. Anti-Mullerian hormone causes the regression of Mullerian ducts which would otherwise differentiate into the uterus and fallopian tubes. Some mutations in the anti-Mullerian hormone result in persistent Mullerian duct syndrome. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.

Long Name

Mullerian-inhibiting Substance/Anti-Mullerian Hormone

Alternate Names

AMH

Gene Symbol

AMH

Additional MIS/AMH Products

Product Documents for MIS/AMH Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for MIS/AMH Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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MIS/AMH Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-70639

Key Product Details

Species Reactivity

Applications

Label

Antibody Source

Concentration

View all MIS/AMH Antibodies »

Product Specifications

Immunogen

Clonality

Host

Isotype

Theoretical MW

Description

Scientific Data Images for MIS/AMH Antibody

Western Blot: MIS/AMH Antibody [NBP1-70639]

Immunohistochemistry: MIS/AMH Antibody [NBP1-70639]

Western Blot: MIS/AMH Antibody [NBP1-70639]

Applications for MIS/AMH Antibody

Immunohistochemistry

Immunohistochemistry-Paraffin

Western Blot

Formulation, Preparation, and Storage

Purification

Formulation

Preservative

Concentration

Shipping

Stability & Storage

Background: MIS/AMH

Long Name

Alternate Names

Gene Symbol

Additional MIS/AMH Products

Product Documents for MIS/AMH Antibody

Product Datasheets

COA

Certificate of Analysis

SDS

Product Specific Notices for MIS/AMH Antibody