MIS12 Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NB100-2358
Conjugate
Catalog #
Key Product Details
Species Reactivity
Validated:
Human
Applications
Immunoprecipitation, Western Blot (Negative)
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
1.0 mg/ml
Product Summary for MIS12 Antibody
Immunogen
The immunogen recognized by this antibody maps to a region between residue 1 and 50 of human MIS12 homolog using the numbering given in entry NP_076944.1 (GeneID 79003).
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for MIS12 Antibody
Immunoprecipitation: MIS12 Antibody [NB100-2358]
Immunoprecipitation: MIS12 Antibody [NB100-2358] - Detection of human MIS12 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-MIS12 antibody NB100-2358 used for IP at 3 ug per reaction. MIS12 was also immunoprecipitated by rabbit anti-MIS12 antibody NB100-2359. For blotting immunoprecipitated MIS12, NB100-2359 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.Applications for MIS12 Antibody
Application
Recommended Usage
Immunoprecipitation
2-5 ug/mg lysate
Application Notes
Immunoprecipitation Not recommended for Western Blot. Use rabbit anti-MIS12 antibody Cat. No. NB 100-2359.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
Tris-Citrate/Phosphate (pH 7.0 - 8.0)
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C. Do not freeze.
Background: MIS12
Alternate Names
2510025F08Rik, hMis12, homolog of yeast Mis12, KNTC2AP, MGC2488, MIS12 homolog, MIS12, MIND kinetochore complex component, homolog (S. pombe), MIS12, MIND kinetochore complex component, homolog (yeast), MTW1, protein MIS12 homolog
Entrez Gene IDs
79003 (Human)
Gene Symbol
MIS12
UniProt
Additional MIS12 Products
Product Documents for MIS12 Antibody
Product Specific Notices for MIS12 Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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