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MIS18A Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-88944

Novus Biologicals, part of Bio-Techne
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NBP1-88944

Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Human

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: MAGVRSLRCSRGCAGGCECGDKGKCSDSSLLGKRLSEDSSRHQLLQKWASMWSSMSEDASVADMERAQLEEEAAAAEERPLVF

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for MIS18A Antibody

Immunohistochemistry-Paraffin: MIS18A Antibody [NBP1-88944]

Immunohistochemistry-Paraffin: MIS18A Antibody [NBP1-88944]

Immunohistochemistry-Paraffin: MIS18A Antibody [NBP1-88944] - Staining in human testis and pancreas tissues using anti-MIS18A antibody. Corresponding MIS18A RNA-seq data are presented for the same tissues.
Western Blot: MIS18A Antibody [NBP1-88944]

Western Blot: MIS18A Antibody [NBP1-88944]

Western Blot: MIS18A Antibody [NBP1-88944] - Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10
Lane 2: Human cell line NTERA-2
Immunocytochemistry/ Immunofluorescence: MIS18A Antibody [NBP1-88944]

Immunocytochemistry/ Immunofluorescence: MIS18A Antibody [NBP1-88944]

Immunocytochemistry/Immunofluorescence: MIS18A Antibody [NBP1-88944] - Staining of human cell line U-2 OS shows localization to nucleus & cytosol. Antibody staining is shown in green.

Applications for MIS18A Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:20 - 1:50

Immunohistochemistry-Paraffin

1:20 - 1:50

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: MIS18A

Alternate Names

B28, C21orf45, C21orf46, FAPP1-associated protein 1, FASP1, hMis18alpha, MIS18 kinetochore protein homolog A (S. pombe), MIS18alpha, protein Mis18-alpha

Gene Symbol

MIS18A

Additional MIS18A Products

Product Documents for MIS18A Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for MIS18A Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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