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MISP1 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-14390

Novus Biologicals, part of Bio-Techne
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NBP2-14390
NBP2-14390-25ul

Key Product Details

Validated by

Orthogonal Validation, Independent Antibodies

Species Reactivity

Human

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

This antibody was developed against a recombinant protein corresponding to the amino acids: SQASGITGSYSVSESPFFSPIHLHSNVAWTVEDPVDSAPPGQRKKEQWYAGINPSDGINSEVLEAIRVTRHKNAMAERWESRIYASEEDD

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for MISP1 Antibody

Western Blot: MISP1 Antibody [NBP2-14390]

Western Blot: MISP1 Antibody [NBP2-14390]

Western Blot: MISP1 Antibody [NBP2-14390] - Analysis in control (vector only transfected HEK293T lysate) and MISP over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).
Immunocytochemistry/ Immunofluorescence: MISP1 Antibody [NBP2-14390]

Immunocytochemistry/ Immunofluorescence: MISP1 Antibody [NBP2-14390]

Immunocytochemistry/Immunofluorescence: MISP1 Antibody [NBP2-14390] - Staining of human cell line U-2 OS shows localization to plasma membrane and focal adhesion sites.
Immunohistochemistry-Paraffin: MISP1 Antibody [NBP2-14390]

Immunohistochemistry-Paraffin: MISP1 Antibody [NBP2-14390]

Immunohistochemistry-Paraffin: MISP1 Antibody [NBP2-14390] - Staining of human liver shows no positivity in hepatocytes as expected.

Applications for MISP1 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:2500 - 1:5000

Immunohistochemistry-Paraffin

1:200-1:500

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: MISP1

Alternate Names

chromosome 19 open reading frame 21, DKFZp686H18209, hypothetical protein LOC126353

Gene Symbol

MISP

Additional MISP1 Products

Product Documents for MISP1 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for MISP1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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