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MIST1 Antibody (6E8/A12/C11P1)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-22478

Novus Biologicals, part of Bio-Techne
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NBP2-22478
NBP2-22478-0.025mg

Key Product Details

Species Reactivity

Validated:

Human, Mouse

Cited:

Mouse

Applications

Validated:

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Western Blot

Cited:

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 6E8/A12/C11P1

Concentration

1 mg/ml

Product Specifications

Immunogen

Mouse Mist1, C-terminus residues 175-197.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for MIST1 Antibody (6E8/A12/C11P1)

Immunohistochemistry: MIST1 Antibody (6E8/A12/C11P1) [NBP2-22478]

Immunohistochemistry: MIST1 Antibody (6E8/A12/C11P1) [NBP2-22478]

Immunohistochemistry: MIST1 Antibody (6E8/A12/C11P1) [NBP2-22478] - Mist1 expression can be observed in mouse gastric chief cells(red). image is courtesy of customer review.
Immunohistochemistry-Paraffin: MIST1 Antibody (6E8/A12/C11P1) [NBP2-22478]

Immunohistochemistry-Paraffin: MIST1 Antibody (6E8/A12/C11P1) [NBP2-22478]

Immunohistochemistry-Paraffin: MIST1 Antibody (6E8/A12/C11P1) [NBP2-22478] - Staining of mouse pancreas tissues. The antibody gives nuclear staining in acinar cells but not in duct or islet cells.

Applications for MIST1 Antibody (6E8/A12/C11P1)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:10-1:500

Immunohistochemistry

1:10 - 1:500

Immunohistochemistry-Frozen

1:10 - 1:500

Immunohistochemistry-Paraffin

1:10 - 1:500

Western Blot

1:100 - 1:500
Application Notes
The antibody gives nuclear staining in acinar cells but not in duct or islet cells. IHC reported in a verified customer review.

Reviewed Applications

Read 1 review rated 5 using NBP2-22478 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS with 1 mg/ml BSA

Preservative

0.05% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: MIST1

MIST1 plays a role in controlling the transcriptional activity of MYOD1, ensuring that expanding myoblastpopulations remain undifferentiated. Repression may occur through muscle-specific E-box occupancy by homodimers. Mayalso negatively regulate bHLH-mediated t

Alternate Names

basic helix-loop-helix domain containing, class B, 8, basic helix-loop-helix family, member a15, bHLHa15, bHLHb8, class A basic helix-loop-helix protein 15, Class B basic helix-loop-helix protein 8, class II bHLH protein MIST1, MIST1MIST-1, Muscle, intestine and stomach expression 1

Entrez Gene IDs

168620 (Human)

Gene Symbol

BHLHA15

UniProt

Additional MIST1 Products

Product Documents for MIST1 Antibody (6E8/A12/C11P1)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for MIST1 Antibody (6E8/A12/C11P1)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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