MIST1 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-30979

Novus Biologicals, part of Bio-Techne
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NBP2-30979
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Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Human

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all MIST1 Antibodies »

Product Specifications

Immunogen

This antibody was developed against a recombinant protein corresponding to amino acids: AKNYIKSLTATILTMSSSRLPGLEGPGPKLYQHYQQQQQVAGGALGATEAQPQGHLQRYSTQIHSFREG

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (80%), Rat (80%)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for MIST1 Antibody

Immunocytochemistry/ Immunofluorescence: MIST1 Antibody [NBP2-30979]

Immunocytochemistry/ Immunofluorescence: MIST1 Antibody [NBP2-30979]

Immunocytochemistry/Immunofluorescence: MIST1 Antibody [NBP2-30979] - Staining of human cell line Hep G2 shows localization to nucleoplasm & the Golgi apparatus.
Immunohistochemistry-Paraffin: MIST1 Antibody [NBP2-30979]

Immunohistochemistry-Paraffin: MIST1 Antibody [NBP2-30979]

Immunohistochemistry-Paraffin: MIST1 Antibody [NBP2-30979] - Staining in human pancreas and skeletal muscle tissues . Corresponding BHLHA15 RNA-seq data are presented for the same tissues.
Immunohistochemistry-Paraffin: MIST1 Antibody [NBP2-30979]

Immunohistochemistry-Paraffin: MIST1 Antibody [NBP2-30979]

Immunohistochemistry-Paraffin: MIST1 Antibody [NBP2-30979] - Staining of human pancreas shows strong nuclear positivity in exocrine glandular cells.
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Applications for MIST1 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:500 - 1:1000

Immunohistochemistry-Paraffin

1:50 - 1:200
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: MIST1

MIST1 plays a role in controlling the transcriptional activity of MYOD1, ensuring that expanding myoblastpopulations remain undifferentiated. Repression may occur through muscle-specific E-box occupancy by homodimers. Mayalso negatively regulate bHLH-mediated t

Alternate Names

basic helix-loop-helix domain containing, class B, 8, basic helix-loop-helix family, member a15, bHLHa15, bHLHb8, class A basic helix-loop-helix protein 15, Class B basic helix-loop-helix protein 8, class II bHLH protein MIST1, MIST1MIST-1, Muscle, intestine and stomach expression 1

Gene Symbol

BHLHA15

UniProt

Q7RTS1 (Human)

Additional MIST1 Products

Product Documents for MIST1 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for MIST1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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