MITF Antibody (21D1418) - Azide Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-33229

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 21D1418

Format

Azide Free

Concentration

1.0 mg/ml

View all MITF Antibodies »

Product Summary for MITF Antibody (21D1418) - Azide Free

Immunogen

A synthetic peptide mapping to the 300-350 a.a. region of accession number O75030.

Specificity

Mitf produces, by alternative splicing, 10 different transcripts, all with introns, putatively encoding 10 different protein isoforms. Therefore, observed molecular weights may vary depending on MITF isoforms expressed. Users are encouraged to utilize the peptide immunogen sequence to gain further information about the isoforms recognized by the antibody.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for MITF Antibody (21D1418) - Azide Free

Western Blot: MITF Antibody (21D1418)Azide Free [NBP2-33229]

Western Blot: MITF Antibody (21D1418)Azide Free [NBP2-33229]

Western Blot: MITF Antibody (21D1418) - Azide Free [NBP2-33229] - Analysis of HeLa lysate (35ug per lane, RIPA buffer) using MiTF antibody (NB100-56561) at 0.1ug/ml. Band detected at ~60kDa. (Expected MW of 58.2kDa according to NP_937802.1).
Immunohistochemistry-Paraffin: MITF Antibody (21D1418) - Azide Free [NBP2-33229]

Immunohistochemistry-Paraffin: MITF Antibody (21D1418) - Azide Free [NBP2-33229]

Immunohistochemistry-Paraffin: MITF Antibody (21D1418) - Azide Free [NBP2-33229] - MITF Antibody (21D1418) [NB100-56561] - Analysis of a FFPE tissue section of human skin using 1:200 dilution of MITF (21D1418) antibody. The staining was developed using HRP labeled anti-rabbit secondary antibody and DAB reagent, and nuclei of cells were counter-stained with hematoxylin. General cytoplasmic staining appears enriched in melanocytes below the epidermis. Some nuclear reactivity is observed as well.
Western Blot: MITF Antibody (21D1418)Azide Free [NBP2-33229]

Western Blot: MITF Antibody (21D1418)Azide Free [NBP2-33229]

Western Blot: MITF Antibody (21D1418) - Azide Free [NBP2-33229] - Analysis of A375 lysate using MITF antibody at 4 ug/ml.

Applications for MITF Antibody (21D1418) - Azide Free

Application
Recommended Usage

Immunohistochemistry

1:200

Immunohistochemistry-Paraffin

1:200

Western Blot

0.1-5ug/ml
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS - BSA free

Format

Azide Free

Preservative

No Preservative

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: MITF

MITF (microphthalmia-associated transcription factor) is a melanocytic nuclear protein that contains basic helix-loop-helix (HLH) and leucine zipper (LZ) domains. These protein motifs are frequently observed in other transcription factors and are particularly common to members of the Myc family. MITF can directly associate with DNA as a homodimer. It is required for the development and differentiation of melanocytes. Its expression is upregulated by cAMP and cAMP dependent pathways. MITF activates several different gene promoters by binding to their E-boxes. Tyrosinase, TRP-1 and TRP-2 are pigment synthesis genes activated by MITF. When MITF is phosphorylated on Serine 73 (via the MAPK pathway), it associates with coactivators of the p300/CBP family and enhances transcription. MITF has several isoforms including MITF-M which is specifically expressed in melanocytes. In Mitfdeficient mice there is a complete absence of melanocytes.

Long Name

Microphthalmia-associated Transcription Factor

Alternate Names

bHLHe32, MI, WS2A

Gene Symbol

MITF

Additional MITF Products

Product Documents for MITF Antibody (21D1418) - Azide Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for MITF Antibody (21D1418) - Azide Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Privacy and Cookie Policy
Site Map
© Copyright 2024 Bio-Techne. All Rights Reserved.