MITF Antibody (C5/D5 Cocktail)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-66627

Novus Biologicals, part of Bio-Techne
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NBP2-66627
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Gel Super Shift Assays, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1/IgG1 Clone # C5/D5 Cocktail

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all MITF Antibodies »

Product Specifications

Immunogen

Hybridoma produced by the fusion of splenocytes from RBF/DnJ mice immunized with an N-terminal fragment of human microphthalmia protein and mouse myeloma NS1 cells.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1/IgG1

Scientific Data Images for MITF Antibody (C5/D5 Cocktail)

Immunohistochemistry-Paraffin: MITF Antibody (C5/D5 Cocktail) [NBP2-66627]

Immunohistochemistry-Paraffin: MITF Antibody (C5/D5 Cocktail) [NBP2-66627]

Immunohistochemistry-Paraffin: MITF Antibody (C5/D5 Cocktail) [NBP2-66627] - Immunohistochemical staining using microphthalmia antibody on formalin fixed, paraffin embedded human melanoma.

Applications for MITF Antibody (C5/D5 Cocktail)

Application
Recommended Usage

Immunohistochemistry-Frozen

1:10 - 1:500

Immunoprecipitation

2 ug/mg protein lysate

Western Blot

1 ug/ml
Application Notes
In Western blotting, it recognizes a doublet of 52-56kDa, identified as serine-phosphorylated and unphosphorylated forms of melanocytic isoforms of microphthalmia (Mi) transcription factor.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

PBS

Preservative

0.08% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: MITF

In Western blotting, it recognizes a doublet of 52-56kDa, identified as serine-phosphorylated and unphosphorylated forms of melanocytic isoforms of microphthalmia (Mi) transcription factor. There are two known isoforms of MiTF differing by 66 amino acids at the NH2 terminus. Shorter forms are expressed in melanocytes and run as two bands at 52kDa and 56kDa, while the longer Mi form runs as a cluster of bands at 60-70kDa in osteoclasts and in B16 melonoma cells (but not other melanoma cell lines), as well as mast cells and heart. It reacts with both melanocytic as well as the non- melanocytic isoforms of MiTF. This Ab does not cross-react with other b-HLH-ZIP factors by DNA mobility shift assay. Mi is a basic helix-loop-helix-leucin zipper (b-HLH-ZIP) transtripotion factor implicated in pigmentation, mast cells and bone development. The mutation of MiTF causes Waardenburg Syndrome type II in humans. In mice, a profound loss of pigmented cells in the skin eye and inner ear results, as well as osteopetrosis and defects in natural killer and mast cells. These melanocyte isoforms have been shown by two dimensional tryptic mapping to differ in c-Kit-induced phosphorylation. Osteopetrotic rat strain harbors a large genomic deletion encompassing the 3Õ half of MiTF including most of the b-HLH-ZIP region. Osteoclasts from these animals lack MiTF protein in contrast to wild-type rat, mouse, and human osteoclasts.

Long Name

Microphthalmia-associated Transcription Factor

Alternate Names

bHLHe32, MI, WS2A

Gene Symbol

MITF

UniProt

O75030 (Human)

Additional MITF Products

Product Documents for MITF Antibody (C5/D5 Cocktail)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for MITF Antibody (C5/D5 Cocktail)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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