MITF Antibody (JF100-01)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-66951

Recombinant Monoclonal Antibody.
Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Applications

Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # JF100-01

Concentration

1 mg/ml

View all MITF Antibodies »

Product Summary for MITF Antibody (JF100-01)

Immunogen

Recombinant protein within Human MITF aa 353-520 / 526. (SwissProt: O75030 Human; SwissProt: Q08874 Mouse; SwissProt: O88368 Rat)

Localization

Nucleus, Cytoplasm.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for MITF Antibody (JF100-01)

Western Blot: MITF Antibody (JF100-01) [NBP2-66951]

Western Blot: MITF Antibody (JF100-01) [NBP2-66951]

Western Blot: MITF Antibody (JF100-01) [NBP2-66951] - Western blot analysis of MITF on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: PC-12 cell lysate Lane 2: A431 cell lysate
Immunocytochemistry/ Immunofluorescence: MITF Antibody (JF100-01) [NBP2-66951]

Immunocytochemistry/ Immunofluorescence: MITF Antibody (JF100-01) [NBP2-66951]

Immunocytochemistry/Immunofluorescence: MITF Antibody (JF100-01) [NBP2-66951] - Staining MiTF in NIH/3T3 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Flow Cytometry: MITF Antibody (JF100-01) [NBP2-66951]

Flow Cytometry: MITF Antibody (JF100-01) [NBP2-66951]

Flow Cytometry: MITF Antibody (JF100-01) [NBP2-66951] - Analysis of SW480 cells with MiTF antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.
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Applications for MITF Antibody (JF100-01)

Application
Recommended Usage

Flow Cytometry

1:50-1:100

Immunocytochemistry/ Immunofluorescence

1:50-1:100

Western Blot

1:500-1:2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

TBS (pH7.4), 0.05% BSA, 40% Glycerol

Preservative

0.05% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: MITF

MITF (microphthalmia-associated transcription factor) is a melanocytic nuclear protein that contains basic helix-loop-helix (HLH) and leucine zipper (LZ) domains. These protein motifs are frequently observed in other transcription factors and are particularly common to members of the Myc family. MITF can directly associate with DNA as a homodimer. It is required for the development and differentiation of melanocytes. Its expression is upregulated by cAMP and cAMP dependent pathways. MITF activates several different gene promoters by binding to their E-boxes. Tyrosinase, TRP-1 and TRP-2 are pigment synthesis genes activated by MITF. When MITF is phosphorylated on Serine 73 (via the MAPK pathway), it associates with coactivators of the p300/CBP family and enhances transcription. MITF has several isoforms including MITF-M which is specifically expressed in melanocytes. In Mitfdeficient mice there is a complete absence of melanocytes.

Long Name

Microphthalmia-associated Transcription Factor

Alternate Names

bHLHe32, MI, WS2A

Gene Symbol

MITF

Additional MITF Products

Product Documents for MITF Antibody (JF100-01)

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Certificate of Analysis

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Product Specific Notices for MITF Antibody (JF100-01)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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