Mitochondrial ribonuclease P protein 3 Antibody

Western Blot: Mitochondrial ribonuclease P protein 3 Antibody [NBP3-12679]

Western Blot: Mitochondrial ribonuclease P protein 3 Antibody [NBP3-12679] - Lane A: 293 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.Developed using the ECL technique.Performed under reducing conditions.Predicted band size:67 kDaObserved band size:67 kDa

Immunocytochemistry/ Immunofluorescence: Mitochondrial ribonuclease P protein 3 Antibody [NBP3-12679]

Immunocytochemistry/Immunofluorescence: Mitochondrial ribonuclease P protein 3 Antibody [NBP3-12679] - Staining of MRPP3 in U2OS cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS,blocked with 10% serum, and incubated with rabbit anti-Human MRPP3 polyclonal antibody (dilution ratio 1:200) at 4? overnight. Then cells were stained with the Alexa Fluor®488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue).Positive staining was localized to Cytoplasm.

Immunoprecipitation: Mitochondrial ribonuclease P protein 3 Antibody [NBP3-12679]

Immunoprecipitation: Mitochondrial ribonuclease P protein 3 Antibody [NBP3-12679] - Lane A:0.5 mg 293 Whole Cell Lysate4 uL anti-MRPP3 rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G.Primary antibody:Anti-MRPP3 rabbit polyclonal antibody,at 1:100 dilution Secondary antibody:Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilutionDeveloped using the ECL technique.Performed under reducing conditions.Predicted band size: 67 kDaObserved band size :67 kDa