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Key Product Details

Species Reactivity

Human, Mouse, Rat, Canine, Monkey

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # OTI1E5

Format

Azide and BSA Free

Concentration

LYOPH mg/ml

Product Specifications

Immunogen

Full length human recombinant protein of human MRPL11(NP_057134) produced in E.coli.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

20.5 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images

Western Blot: Mitochondrial ribosomal protein L11 Antibody (OTI1E5)Azide and BSA Free [NBP2-72707]

Western Blot: Mitochondrial ribosomal protein L11 Antibody (OTI1E5)Azide and BSA Free [NBP2-72707]

Western Blot: Mitochondrial ribosomal protein L11 Antibody (OTI1E5) - Azide and BSA Free [NBP2-72707] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Mitochondrial ribosomal protein L11.
Western Blot: Mitochondrial ribosomal protein L11 Antibody (OTI1E5)Azide and BSA Free [NBP2-72707]

Western Blot: Mitochondrial ribosomal protein L11 Antibody (OTI1E5)Azide and BSA Free [NBP2-72707]

Western Blot: Mitochondrial ribosomal protein L11 Antibody (OTI1E5) - Azide and BSA Free [NBP2-72707] - Analysis of extracts (35ug) from 9 different cell lines (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).

Applications

Application
Recommended Usage

Immunohistochemistry

1:150

Western Blot

1:2000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Reconstitution

we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Formulation

Lyophilized from PBS (pH 7.3) with 8% Trehalose

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Mitochondrial ribosomal protein L11

MRPL11, also known as 39 S ribosomal protein L11, mitochondrial, consists of a 20.7 kDa, a 18.2 kDa, and a 19.5 kDa isoform and is utilized in mitochondrial ribosomes during protein synthesis. Diseases and disorders such as pneumonia, malaria, mycobacterium tuberculosis, and intrahepatic cholangiocarcinoma are being research with this protein. The protein interacts in the translational control pathway with ICT1, RPL26, MRPL2, RPL26L1, and MRPL3.

Alternate Names

L11MT, MGC111024, mitochondrial ribosomal protein L11, MRP-L11,39S ribosomal protein L11, mitochondrial

Gene Symbol

MRPL11

Additional Mitochondrial ribosomal protein L11 Products

Product Documents

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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