mitochondrial ribosomal protein L4 Antibody (4B1)

Novus Biologicals, part of Bio-Techne | Catalog # H00051073-M02

Novus Biologicals, part of Bio-Techne
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H00051073-M02
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Key Product Details

Species Reactivity

Human

Applications

ELISA, Sandwich ELISA

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2a Kappa Clone # 4B1

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all mitochondrial ribosomal protein L4 Antibodies »

Product Specifications

Immunogen

MRPL4 (AAH00756, 1 a.a. ~ 263 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MLQFVRAGARAWLRPTGSQGLSSLAEEAARATENPEQVASEGLPEPVLRKVELPVPTHRRPVQAWVESLRGFEQERVGLADLHPDVFATAPRLDILHQVAMWQKNFKRISYAKTKTRAEVRGGGRKPWPQKGTGRARHGSIRSPLWRGGGVAHGPRGPTSYYYMLPMKVRALGLKVALTVKLAQDDLHIMDSLELPTGDPQYLTELAHYRRWGDSVLLVDLTHEEMPQSIVEATSRLKTFNLIPAVGEQRAQAPRVRMCRLRC

Specificity

Reacts with mitochondrial ribosomal protein L4.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Scientific Data Images for mitochondrial ribosomal protein L4 Antibody (4B1)

Sandwich ELISA: mitochondrial ribosomal protein L4 Antibody (4B1) [H00051073-M02] - Detection limit for recombinant GST tagged MRPL4 is 3 ng/ml as a capture antibody.

Applications for mitochondrial ribosomal protein L4 Antibody (4B1)

Application
Recommended Usage

ELISA

Optimal dilutions of this antibody should be experimentally determined.

Sandwich ELISA

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
This antibody is reactive against recombinant protein in ELISA.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

In 1x PBS, pH 7.4

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: mitochondrial ribosomal protein L4

Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 39S subunit protein. Sequence analysis identified alternatively spliced variants that encode different protein isoforms. [provided by RefSeq]

Alternate Names

CGI-28, L4mt, MGC16367, MGC2681, mitochondrial ribosomal protein L4,39S ribosomal protein L4, mitochondrial, MRP-L4

Entrez Gene IDs

51073 (Human)

Gene Symbol

MRPL4

UniProt

AAH00756 (Human)

Additional mitochondrial ribosomal protein L4 Products

Product Documents for mitochondrial ribosomal protein L4 Antibody (4B1)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for mitochondrial ribosomal protein L4 Antibody (4B1)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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