Mitofilin Antibody (M027)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-23409
Key Product Details
Species Reactivity
Human
Applications
Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # M027
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
Clone M027 was generated from a proprietary antigen related to human mitofilin expressed in the A431 epidermoid carcinoma cell line. [UniProt# Q16891]
Specificity
Clone M027 detects a 90 kDa band corresponding to the molecular mass of mitofilin on SDS-PAGE immunoblots of native or denatured human PC3, MDA-MB-231, A431, A549, and MeWo cell lysates, as well as human breast, skin, and brain tissues. In addition, mass spectrometry analysis of immunoprecipitates using MM0271 in human A431 cell lysates confirms this antibody only detects Mitofilin protein.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Theoretical MW
90 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Description
Stable for at least 1 year at -20°C.
Scientific Data Images for Mitofilin Antibody (M027)
Immunocytochemistry/Immunofluorescence: Mitofilin Antibody (M027) [NBP3-23409] -
Immunocytochemistry/Immunofluorescence: Mitofilin Antibody (M027) [NBP3-23409] - Immunocytochemical labeling of mitofilin in methanol-acetone (1:1) fixed human MeWo cells. The cells were labeled with mouse monoclonal anti-mitofilin (clone M027). The antibody was detected using goat anti-mouse DyLight(R) 594.
Immunocytochemistry/Immunofluorescence: Mitofilin Antibody (M027) [NBP3-23409] -
Immunocytochemistry/Immunofluorescence: Mitofilin Antibody (M027) [NBP3-23409] - Immunocytochemical labeling of mitofilin in methanol-acetone (1:1) fixed human MeWo cells. The cells were labeled with mouse monoclonal anti-mitofilin (clone M027). The antibody was detected using goat anti-mouse DyLight(R) 594.
Western Blot: Mitofilin Antibody (M027) [NBP3-23409] -
Western Blot: Mitofilin Antibody (M027) [NBP3-23409] - Western blot analysis of human cell lysates: MeWo (lane 1), MDA-MB-231 (lane 2), PC3 (lane 3), and A549 (lane 4). The blot was probed with mouse monoclonal anti-mitofilin (NBP3-23409) at 1:1000.Applications for Mitofilin Antibody (M027)
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:100
Immunoprecipitation
1:100
Western Blot
1:1000
Formulation, Preparation, and Storage
Purification
Protein G purified
Formulation
PBS, 1 mg/ml BSA and 50% glycerol
Preservative
0.05% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C.
Background: Mitofilin
Alternate Names
DKFZp779P1653, inner membrane protein, mitochondrial, MGC111146, Mic 60, Mic60, MICOS Complex Subunit MIC60, mitochondrial (mitofilin), Mitofilin, motor protein, p87/89, proliferation-inducing gene 4
Gene Symbol
IMMT
Additional Mitofilin Products
Product Documents for Mitofilin Antibody (M027)
Product Specific Notices for Mitofilin Antibody (M027)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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