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MKK3/MKK6 Antibody (SR1645)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-21693

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
Catalog #
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NBP3-21693-100ul
NBP3-21693-50ul

Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Applications

Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # SR1645

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Product Summary for MKK3/MKK6 Antibody (SR1645)

Immunogen

A synthesized peptide derived from human MKK3/MKK6 (Uniprot #: P46734, P52564)

Specificity

Detects endogenous levels of MKK3/MKK6

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for MKK3/MKK6 Antibody (SR1645)

Immunohistochemistry: MKK3/MKK6 Antibody (SR1645) [NBP3-21693] -

Immunohistochemistry: MKK3/MKK6 Antibody (SR1645) [NBP3-21693] - Immunohistochemical analysis of paraffin-embedded mouse spleen, using NBP3-21693.

Immunocytochemistry/Immunofluorescence: MKK3/MKK6 Antibody (SR1645) [NBP3-21693] -

Immunocytochemistry/Immunofluorescence: MKK3/MKK6 Antibody (SR1645) [NBP3-21693] - Immunofluorescent analysis of Hela cells, using NBP3-21693.

Western Blot: MKK3/MKK6 Antibody (SR1645) [NBP3-21693] -

Western Blot: MKK3/MKK6 Antibody (SR1645) [NBP3-21693] - Western blot analysis using NBP3-21693 in HeLa cell lysate.

Applications for MKK3/MKK6 Antibody (SR1645)

Application
Recommended Usage

Flow Cytometry

1:50

Immunocytochemistry/ Immunofluorescence

1:100-1:500

Immunohistochemistry

1:50-1:200

Immunoprecipitation

1:50

Western Blot

1:500-1:1000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, pH 7.4, 150mM NaCl, 50% glycerol.

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: MKK3/MKK6

Mitogen-activated protein kinase kinase 3 (MKK3), also known as MEK3, and the closely related MKK6 are activated by proinflammatory cytokines and environmental stresses. Activation occurs through phosphorylation by several upstream MAPK kinase kinases, including ASK1 and TAK1. Both MKK3 and MKK6 are dual specificity protein kinases, phosphorylating and activating the p38 MAP kinases at Thr and Tyr positions within the phosphoacceptor sequence Thr-Gly-Tyr.

Long Name

Mitogen-activated Protein Kinase Kinase 3

Alternate Names

Dual specificity mitogen-activated protein kinase kinase 6;MAP2K6;MAPKK6;MEK6;MKK6;PRKMK6;SAPKK3;SAPKK-3

Gene Symbol

MAP2K6

Additional MKK3/MKK6 Products

Product Documents for MKK3/MKK6 Antibody (SR1645)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for MKK3/MKK6 Antibody (SR1645)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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