MKK4/MEK4 Antibody - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-03652

Novus Biologicals, part of Bio-Techne
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NBP3-03652-100ul
NBP3-03652-20ul
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Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Knockout Validated, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all MKK4 Antibodies »

Product Specifications

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 300-399 of human MKK4/MEK4 (NP_003001.1). LATGRFPYPKWNSVFDQLTQVVKGDPPQLSNSEEREFSPSFINFVNLCLTKDESKRPKYKELLKHPFILMYEERAVEVACYVCKILDQMPATPSSPMYVD

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for MKK4/MEK4 Antibody - BSA Free

Western Blot: MKK4/MEK4 AntibodyBSA Free [NBP3-03652]

Western Blot: MKK4/MEK4 AntibodyBSA Free [NBP3-03652]

Western Blot: MKK4/MEK4 Antibody [NBP3-03652] - Analysis of extracts of various cell lines, using MKK4/MEK4 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit.
Knockout Validated: MKK4/MEK4 Antibody - BSA Free [NBP3-03652]

Knockout Validated: MKK4/MEK4 Antibody - BSA Free [NBP3-03652]

Knockout Validated: MKK4/MEK4 Antibody [NBP3-03652] - Analysis of extracts from normal (control) and MAP2K4 knockout (KO) HeLa cells, using MKK4/MEK4 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.

Applications for MKK4/MEK4 Antibody - BSA Free

Application
Recommended Usage

Immunohistochemistry

1:50-1:100

Western Blot

1:500-1:2000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS with 50% glycerol, pH7.3.

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: MKK4

MKK4, also known as JNKK1 and SEK1, is a dual specificity kinase that belongs to the Ser/Thr protein kinase family. MKK4 is a direct activator of MAP kinases in response to various environmental stresses or mitogenic stimuli. MKK4 phosphorylates and activates p38 MAP kinase and JNK but not ERK 1/2 (1-2). JNK is activated by dual phosphorylation at Thr and Tyr by MKK4 and MKK7; although MKK4 will preferentially phosphorylate Tyr (3). MKK4 is also known to be a tumor suppressor, as mutations inactivating the MKK4 gene have been found in 5% of a wide variety of tumor types (4).

Long Name

Mitogen-activated Protein Kinase Kinase 4

Alternate Names

JNKK1, MAP2K4, MAPKK4, MEK4, PRKMK4, SERK1

Gene Symbol

MAP2K4

Additional MKK4 Products

Product Documents for MKK4/MEK4 Antibody - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for MKK4/MEK4 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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