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MKRN1 Antibody (OTI2C8) - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-72718

Novus Biologicals, part of Bio-Techne
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NBP2-72718
Conjugate
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Key Product Details

Species Reactivity

Human, Mouse

Applications

CyTOF-ready, Flow Cytometry, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # OTI2C8

Format

Azide and BSA Free

Concentration

LYOPH mg/ml

Product Specifications

Immunogen

Full length human recomnbinant protein of human MKRN1(NP_038474) produced in HEK293T cell.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Theoretical MW

53.2 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for MKRN1 Antibody (OTI2C8) - Azide and BSA Free

Western Blot: MKRN1 Antibody (OTI2C8)Azide and BSA Free [NBP2-72718]

Western Blot: MKRN1 Antibody (OTI2C8)Azide and BSA Free [NBP2-72718]

Western Blot: MKRN1 Antibody (OTI2C8) - Azide and BSA Free [NBP2-72718] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MKRN1.
Flow Cytometry: MKRN1 Antibody (OTI2C8) - Azide and BSA Free [NBP2-72718]

Flow Cytometry: MKRN1 Antibody (OTI2C8) - Azide and BSA Free [NBP2-72718]

Flow Cytometry: MKRN1 Antibody (OTI2C8) - Azide and BSA Free [NBP2-72718] - Analysis of Jurkat cells, using anti-MKRN1 antibody , (Red), compared to a nonspecific negative control antibody, (Blue).

Applications for MKRN1 Antibody (OTI2C8) - Azide and BSA Free

Application
Recommended Usage

Flow Cytometry

1:100

Immunohistochemistry

1:150

Western Blot

1:1000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Reconstitution

we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Formulation

Lyophilized from PBS (pH 7.3) with 8% Trehalose

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: MKRN1

MKRN1 was identified as a repressor of c-Jun, androgen receptor, and retinoic acid receptor transcriptional activity. MKRN1 is a RING zinc finger protein that has been demonstrated to function as an E3 ubiquitin ligase. The repressive activity of MKRN1 is not dependent on its ubiquitin ligase activity. MKRN1 also appears to function as a transactivator of RNA polymerase II dependent transcription.

Alternate Names

E3 ubiquitin-protein ligase makorin-1, EC 6.3.2.-, FLJ21334, makorin ring finger protein 1, RNF61RING finger protein 61

Gene Symbol

MKRN1

Additional MKRN1 Products

Product Documents for MKRN1 Antibody (OTI2C8) - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for MKRN1 Antibody (OTI2C8) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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