MKRN2 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NB100-55250

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Human, Mouse

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

1.0 mg/ml

View all MKRN2 Antibodies »

Product Specifications

Immunogen

The immunogen recognized by this antibody maps to a region between residue 366 and the C-terminus (residue 416) of human makorin, ring finger protein, 2 using the numbering given in entry NP_054879.3 (GeneID 23609).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for MKRN2 Antibody

Western Blot: MKRN2 Antibody [NB100-55250]

Western Blot: MKRN2 Antibody [NB100-55250]

Western Blot: MKRN2 Antibody [NB100-55250] - Whole cell lysate from HeLa (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded), 293T (T; 50 mcg) and mouse NIH3T3 (M; 50 mcg) cells. NB100-55250 used for WB at 0.1 mcg/ml (A) and 1 mcg/ml (B), and used for IP at 3 mcg/mg lysate (B).
Immunohistochemistry-Paraffin: MKRN2 Antibody [NB100-55250]

Immunohistochemistry-Paraffin: MKRN2 Antibody [NB100-55250]

Immunohistochemistry-Paraffin: MKRN2 Antibody [NB100-55250] - Human breast carcinoma. Antibody: Affinity purified rabbit anti-MKRN2 used at a dilution of 1:1,000 (1ug/ml). Detection: DAB

Applications for MKRN2 Antibody

Application
Recommended Usage

Immunohistochemistry

1:1000 to 1:5000

Immunohistochemistry-Paraffin

1:1000 to 1:5000

Immunoprecipitation

2-5 ug/mg lysate

Western Blot

1:2000-1:10000
Application Notes
Epitope retrieval with Tris-EDTA pH9.0 is recommended for FFPE tissue sections.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: MKRN2

The basic function of MKRN2 is mediating ubiquitin-conjugating enzyme (E2)-dependent ubiquitination. Makorin RING zinc finger-2 (MKRN2), overlaps and is antisense to the gene RAF1 in mammals. Northern blot analyses show that human MKRN2 and RAF1 are co-expressed in tissues and cell lines, raising the possibility of mRNA duplex formation. The encoded makorin-2 protein is likely a ribonucleoprotein of unknown function, but its conservation suggests an important cellular role.

Alternate Names

EC 6.3.2.-, HSPC070, makorin ring finger protein 2, probable E3 ubiquitin-protein ligase makorin-2, RNF62RING finger protein 62

Entrez Gene IDs

23609 (Human); 67027 (Mouse)

Gene Symbol

MKRN2

UniProt

Q9H000 (Human)

Additional MKRN2 Products

Product Documents for MKRN2 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for MKRN2 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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