MLH3 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-78702

Novus Biologicals, part of Bio-Techne
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NBP2-78702
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Key Product Details

Species Reactivity

Validated:

Human

Applications

Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Peptide ELISA

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all MLH3 Antibodies »

Product Summary for MLH3 Antibody

Immunogen

Peptide with sequence RQSLQQSMPPCEPP corresponding to C-Terminus according to NP_001035197.1, NP_055196.2.

Specificity

This antibody is expected to recognize isoform 1 (NP_001035197.1) and isoform 2 (NP_055196.2).

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for MLH3 Antibody

Immunocytochemistry/ Immunofluorescence: MLH3 Antibody [NBP2-78702]

Immunocytochemistry/ Immunofluorescence: MLH3 Antibody [NBP2-78702]

Immunocytochemistry/Immunofluorescence: MLH3 Antibody [NBP2-78702] - Analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL), showing nuclear staining. Actin filaments were stained with phalloidin (red) and the nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL).
Flow Cytometry: MLH3 Antibody [NBP2-78702]

Flow Cytometry: MLH3 Antibody [NBP2-78702]

Flow Cytometry: MLH3 Antibody [NBP2-78702] - Analysis of paraformaldehyde fixed HeLa cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (1 ug/mL). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.

Applications for MLH3 Antibody

Application
Recommended Usage

Flow Cytometry

10 ug/mL

Immunocytochemistry/ Immunofluorescence

10 ug/mL

Peptide ELISA

Detection limit 1:2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: MLH3

This gene is a member of the MutL-homolog (MLH) family of DNA mismatch repair (MMR) genes. MLH genes are implicated in maintaining genomic integrity during DNA replication and after meiotic recombination. The protein encoded by this gene functions as a heterodimer with other family members. Somatic mutations in this gene frequently occur in tumors exhibiting microsatellite instability, and germline mutations have been linked to hereditary nonpolyposis colorectal cancer type 7 (HNPCC7). Several alternatively spliced transcript variants have been identified, but the full-length nature of only two transcript variants has been determined.

Alternate Names

DNA mismatch repair protein Mlh3, HNPCC7, MGC138372, mutL (E. coli) homolog 3, mutL homolog 3 (E. coli), MutL protein homolog 3

Gene Symbol

MLH3

Additional MLH3 Products

Product Documents for MLH3 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for MLH3 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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