MLK3 Antibody (S07-2C3)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-19695

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Human, Mouse

Applications

Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # S07-2C3

Concentration

0.3 mg/ml

View all MLK3 Antibodies »

Product Specifications

Immunogen

A synthetic peptide of human MLK3 (Uniprot # Q16584)

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

93 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for MLK3 Antibody (S07-2C3)

Western Blot: MLK3 Antibody (S07-2C3) [NBP3-19695]

Western Blot: MLK3 Antibody (S07-2C3) [NBP3-19695]

Western Blot: MLK3 Antibody (S07-2C3) [NBP3-19695] - Western blot detection of MLK3 in HepG2, A431 lysates using MLK3 antibody. Predicted band size: 93kDa. Observed band size: 93kDa.

Applications for MLK3 Antibody (S07-2C3)

Application
Recommended Usage

Western Blot

1/2000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.05% BSA

Preservative

0.01% Sodium Azide

Concentration

0.3 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: MLK3

Mixed lineage kinase-3 (MLK-3) is a mitogen-activated kinase kinase kinase that mediates stress-activating protein kinase (SAPK)/c-Jun NH2-terminal kinase activation. MLK-3 and other MLK family kinases are characterized by the presence of multiple protein-protein interaction domains including a tandem leucine/isoleucine zipper (LZs) motif. Leucine zippers are known to mediate protein dimerization raising the possibility that the tandem leucine/isoleucine zippers may function as a dimerization motif of MLK-3 (1). MLK-3 has several interesting structural features including an SH3 domain in the absence of an SH2 domain, a region containing two leucine zippers with an adjacent carboxy-terminal basic region, and a proline rich region. (2). HPK1 has been found to phosphorylate MLK-3 activation loop in vitro, and Ser281 was found to be the major phosphorylation site, indicating that HPK1 also activates MLK-3 via phosphorylation of the kinase activation loop (3).

Long Name

Mixed Lineage Kinase 3

Alternate Names

MAP3K11, PTK1, RHOE, SPRK

Gene Symbol

MAP3K11

Additional MLK3 Products

Product Documents for MLK3 Antibody (S07-2C3)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for MLK3 Antibody (S07-2C3)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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