MLK4 Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP1-41081
![Western Blot: MLK4 Antibody [NBP1-41081]](https://resources.bio-techne.com/images/products/MLK4-Antibody-Western-Blot-NBP1-41081-img0003.jpg "Western Blot: MLK4 Antibody [NBP1-41081]")
Key Product Details
Validated by
Independent Antibodies, Biological Validation
Species Reactivity
Validated:
Human
Cited:
Human
Applications
Validated:
Immunoprecipitation, Western Blot
Cited:
Immunoblotting, Immunoprecipitation, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
0.2 mg/ml
Product Specifications
Immunogen
The immunogen for this product maps to a region between residue 986 and 1036 of human mixed lineage kinase 4 using the numbering given in entry NP_115811.2 (GeneID 84451).
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for MLK4 Antibody
![Western Blot: MLK4 Antibody [NBP1-41081]](https://resources.bio-techne.com/images/products/MLK4-Antibody-Western-Blot-NBP1-41081-img0002.jpg "Western Blot: MLK4 Antibody [NBP1-41081]")
Western Blot: MLK4 Antibody [NBP1-41081]
Western Blot: MLK4 Antibody [NBP1-41081] - Whole cell lysate from HeLa (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded) and 293T (T; 50 ug) cells. Antibodies: Affinity purified rabbit antiMLK4 antibody NBP1-41081 used for WB at 0.04 ug/ml (A) and 1 ug/ml (B) and used for IP at 3 ug/mg lysate. MLK4 was also immunoprecipitated by rabbit anti-MLK4 antibody NBP1-41080, which recognizes an upstream epitope.Applications for MLK4 Antibody
Application
Recommended Usage
Immunoprecipitation
2-5 ug/mg lysate
Western Blot
1:2000-1:10000
Application Notes
Western blot of lysates performed using standard western blot reagents and 4-8% SDS-PAGE
Reviewed Applications
Read 1 review rated 5 using NBP1-41081 in the following applications:
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
TBS and 0.1% BSA
Preservative
0.09% Sodium Azide
Concentration
0.2 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C. Do not freeze.
Background: MLK4
Alternate Names
dJ862P8.3, EC 2.7.11, EC 2.7.11.25, mitogen-activated protein kinase kinase kinase MLK4, mixed lineage kinase 4, MLK4, RP5-862P8.2
Entrez Gene IDs
84451 (Human)
Gene Symbol
MAP3K21
UniProt
Additional MLK4 Products
Product Documents for MLK4 Antibody
Product Specific Notices for MLK4 Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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