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MLK4 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-90950

Novus Biologicals, part of Bio-Techne
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NBP1-90950
NBP1-90950-25ul

Key Product Details

Species Reactivity

Human

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: GNPTPTGATIISATGASALPLCPSPAPHSHLPREVSPKKHSTVHIVPQRRPASLRSRSDLPQAYPQTAVSQLAQTACVVGRPGPHPTQFLAAKERTKSHVPSLLDADVEGQSRDYTVPLCRMRSKTSRPSIYELEKE

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for MLK4 Antibody

Western Blot: MLK4 Antibody [NBP1-90950]

Western Blot: MLK4 Antibody [NBP1-90950]

Western Blot: MLK4 Antibody [NBP1-90950] - Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11
Lane 2: Human cell line RT-4
Lane 3: Human cell line U-251MG sp
Immunocytochemistry/ Immunofluorescence: MLK4 Antibody [NBP1-90950]

Immunocytochemistry/ Immunofluorescence: MLK4 Antibody [NBP1-90950]

Immunocytochemistry/Immunofluorescence: MLK4 Antibody [NBP1-90950] - Immunofluorescent staining of human cell line A-431 shows localization to plasma membrane & cytosol.
Immunohistochemistry-Paraffin: MLK4 Antibody [NBP1-90950]

Immunohistochemistry-Paraffin: MLK4 Antibody [NBP1-90950]

Immunohistochemistry-Paraffin: MLK4 Antibody [NBP1-90950] - Staining of human cerebellum shows strong cytoplasmic positivity in Purkinje cells.

Applications for MLK4 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:50 - 1:200

Immunohistochemistry-Paraffin

1:50 - 1:200

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF, Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: MLK4

Activates the JUN N-terminal pathway

Alternate Names

dJ862P8.3, EC 2.7.11, EC 2.7.11.25, mitogen-activated protein kinase kinase kinase MLK4, mixed lineage kinase 4, MLK4, RP5-862P8.2

Gene Symbol

MAP3K21

Additional MLK4 Products

Product Documents for MLK4 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for MLK4 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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