MLLT1 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-26653

Novus Biologicals, part of Bio-Techne
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NBP1-26653
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Key Product Details

Validated by

Independent Antibodies

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot

Cited:

Immunoprecipitation

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

0.2 mg/ml

View all MLLT1 Antibodies »

Product Specifications

Immunogen

The immunogen recognized by this antibody maps to a region between residue 325 and 375 of human myeloid/lymphoid or mixed-lineage leukemia (trithorax homolog, Drosophila); translocated to, 1 using the numbering given in entry NP_005925.2 (GeneID 4298).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for MLLT1 Antibody

Western Blot: MLLT1 Antibody [NBP1-26653]

Western Blot: MLLT1 Antibody [NBP1-26653]

Western Blot: MLLT1 Antibody [NBP1-26653] - Whole cell lysate from HeLa cells. MLLT1 was also immunoprecipitated by rabbit anti-MLLT1 antibody NBP1-26652.
Immunohistochemistry-Paraffin: MLLT1 Antibody [NBP1-26653]

Immunohistochemistry-Paraffin: MLLT1 Antibody [NBP1-26653]

Immunohistochemistry-Paraffin: MLLT1 Antibody [NBP1-26653] - Sample: FFPE section of human testicular seminoma. Antibody: Affinity purified rabbit anti- MLLT1 used at a dilution of 1:200 (1ug/ml). Detection: DAB

Whole cell lysate (10 µg) from HEK293T, HeLa, and LNCaP cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-MLLT1 antibody used for WB at 0.04 µg/ml. Detection: Chemiluminescencewith an exposure time of 75 seconds.

Whole cell lysate (10 µg) from HEK293T, HeLa, and LNCaP cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-MLLT1 antibody used for WB at 0.04 µg/ml. Detection: Chemiluminescencewith an exposure time of 75 seconds.

Applications for MLLT1 Antibody

Application
Recommended Usage

Immunohistochemistry

1:100 - 1:500

Immunohistochemistry-Paraffin

1:100-1:500

Immunoprecipitation

2 - 10 ug/mg lysate

Western Blot

1:2000-1:10000
Application Notes
Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

TBS and 0.1% BSA

Preservative

0.09% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: MLLT1

MLLT1, also known as ENL (eleven nineteen leukemia protein), was originally identified as a gene involved in a recurrent translocation (11;19) with the mixed-lineage leukemia (MLL) gene. The MLL-ENL fusion protein has been found to associate with SWI/SNF complexes and promote transcription from the HoxA7 promoter. Additionally, from studies to identify proteins that associate with MLLT1, MLLT1 is suggested to play a role in histone modification and transcriptional elongation.

Alternate Names

ENLENL/MLL fusion, LTG19MLLT1/MLL fusion, myeloid/lymphoid or mixed-lineage leukemia (trithorax (Drosophila) homolog);translocated to, 1, myeloid/lymphoid or mixed-lineage leukemia (trithorax homolog, Drosophila);translocated to, 1, protein ENL, YEATS domain-containing protein 1, YEATS1myeloid/lymphoid or mixed-lineage leukemia (trithorax homolog); translocatedto, 1

Entrez Gene IDs

4298 (Human)

Gene Symbol

MLLT1

UniProt

Q03111 (Human)

Additional MLLT1 Products

Product Documents for MLLT1 Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for MLLT1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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