MMACHC Antibody (OTI1A4) - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-72738

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Human

Applications

CyTOF-ready, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # OTI1A4

Format

Azide and BSA Free

Concentration

LYOPH mg/ml

View all MMACHC Antibodies »

Product Specifications

Immunogen

Full length human recombinant protein of human MMACHC(NP_056321) produced in HEK293T cell.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

31.5 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for MMACHC Antibody (OTI1A4) - Azide and BSA Free

Western Blot: MMACHC Antibody (OTI1A4)Azide and BSA Free [NBP2-72738]

Western Blot: MMACHC Antibody (OTI1A4)Azide and BSA Free [NBP2-72738]

Western Blot: MMACHC Antibody (OTI1A4) - Azide and BSA Free [NBP2-72738] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MMACHC cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MMACHC.
Immunocytochemistry/ Immunofluorescence: MMACHC Antibody (OTI1A4) - Azide and BSA Free [NBP2-72738]

Immunocytochemistry/ Immunofluorescence: MMACHC Antibody (OTI1A4) - Azide and BSA Free [NBP2-72738]

Immunocytochemistry/Immunofluorescence: MMACHC Antibody (OTI1A4) - Azide and BSA Free [NBP2-72738] - Analysis of COS7 cells transiently transfected by pCMV6-ENTRY MMACHC.
Flow Cytometry: MMACHC Antibody (OTI1A4) - Azide and BSA Free [NBP2-72738]

Flow Cytometry: MMACHC Antibody (OTI1A4) - Azide and BSA Free [NBP2-72738]

Flow Cytometry: MMACHC Antibody (OTI1A4) - Azide and BSA Free [NBP2-72738] - Analysis of HEK293T cells transfected with either overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostaining by MMACHC antibody.

Applications for MMACHC Antibody (OTI1A4) - Azide and BSA Free

Application
Recommended Usage

Flow Cytometry

1:100

Immunocytochemistry/ Immunofluorescence

1:100

Immunohistochemistry

1:150

Western Blot

1:2000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Reconstitution

we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Formulation

Lyophilized from PBS (pH 7.3) with 8% Trehalose

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: MMACHC

The exact function of the protein encoded by this gene is not known, however, its C-terminal region shows similarity to TonB, a bacterial protein involved in energy transduction for cobalamin (vitamin B12) uptake. Hence, it is postulated that this protein may have a role in the binding and intracellular trafficking of cobalamin. Mutations in this gene are associated with methylmalonic aciduria and homocystinuria type cblC. (provided by RefSeq)

Alternate Names

cblC, DKFZP564I122, FLJ25671, methylmalonic aciduria (cobalamin deficiency) cblC type, with homocystinuria, methylmalonic aciduria and homocystinuria type C protein, RP11-291L19.3

Gene Symbol

MMACHC

Additional MMACHC Products

Product Documents for MMACHC Antibody (OTI1A4) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for MMACHC Antibody (OTI1A4) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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