MNK1 Antibody (5E2)
Novus Biologicals, part of Bio-Techne | Catalog # H00008569-M05
Key Product Details
Species Reactivity
Human
Applications
ELISA, Immunocytochemistry/ Immunofluorescence, Sandwich ELISA, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2a Kappa Clone # 5E2
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
MKNK1 (AAH02755.1, 1 a.a. ~ 465 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MVSSQKLEKPIEMGSSEPLPIADGDRRRKKKRRGRATDSLPGKFEDMYKLTSELLGEGAYAKVQGAVSLQNGKEYAVKIIEKQAGHSRSRVFREVETLYQCQRNKNILELIEFFEDDTRFYLVFEKLQGGSILAHIQKQKHFNEREASRVVRGVAAALDFLHTKDKVSLCHLGWSAMAPSGLTAAPTSLGSSDPPTSASQVAGTTGIAHRDLKPENILCESPEKVSPVKICDFDLGSGMKLNNSCTPITTPELTTPCGSAEYMAPEVVEVFTDQATFYDKRCDLWSLGVVLYIMLSGYPPFVGHCGADCGWDRGEVCRVCQNKLFESIQEGKYEFPDKDWAHISSEAKDLISKLLVRDAKQRLSAAQVLQHPWVQGQAPEKGLPTPQVLQRNSSTMDLTLFAAEAIALNRLLSQHEENELAEEPEALADGLCSMKLSPPCKSRLARRRALAQAGRGEDRSPPTAL
Localization
Isoforms 1 and 2 cytoplasmic, isoform 3 nuclear
Specificity
MKNK1 - MAP kinase interacting serine/threonine kinase 1
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2a Kappa
Description
Quality control test: Antibody Reactive Against Recombinant Protein.
Scientific Data Images for MNK1 Antibody (5E2)
![Western Blot: MNK1 Antibody (5E2) [H00008569-M05]](https://resources.bio-techne.com/images/products/MNK1-Antibody-5E2-Western-Blot-H00008569-M05-img0005.jpg "Western Blot: MNK1 Antibody (5E2) [H00008569-M05]")
Western Blot: MNK1 Antibody (5E2) [H00008569-M05]
Western Blot: MNK1 Antibody (5E2) [H00008569-M05] - Analysis of MKNK1 expression in transfected 293T cell line by MKNK1 monoclonal antibody (M05), clone 5E2. Lane 1: MKNK1 transfected lysatE (51.3 KDa). Lane 2: Non-transfected lysate.![Immunocytochemistry/ Immunofluorescence: MNK1 Antibody (5E2) [H00008569-M05]](https://resources.bio-techne.com/images/products/MNK1-Antibody-5E2-Immunocytochemistry-Immunofluorescence-H00008569-M05-img0003.jpg "Immunocytochemistry/ Immunofluorescence: MNK1 Antibody (5E2) [H00008569-M05]")
Immunocytochemistry/ Immunofluorescence: MNK1 Antibody (5E2) [H00008569-M05]
Immunocytochemistry/Immunofluorescence: MNK1 Antibody (5E2) [H00008569-M05] - Analysis of monoclonal antibody to MKNK1 on HeLa cell. Antibody concentration 10 ug/ml
Sandwich ELISA: MNK1 Antibody (5E2) [H00008569-M05] - Detection limit for recombinant GST tagged MKNK1 is approximately 1ng/ml as a capture antibody.
Applications for MNK1 Antibody (5E2)
Application
Recommended Usage
ELISA
Optimal dilutions of this antibody should be experimentally determined.
Immunocytochemistry/ Immunofluorescence
Optimal dilutions of this antibody should be experimentally determined.
Sandwich ELISA
Optimal dilutions of this antibody should be experimentally determined.
Western Blot
Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Antibody reactivity against Recombinant Protein with GST tag on ELISA and WB. GST tag alone is used as a negative control.
Formulation, Preparation, and Storage
Purification
IgG purified
Formulation
In 1x PBS, pH 7.4
Preservative
No Preservative
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Background: MNK1
Long Name
MAP kinase-interacting serine/threonine-protein kinase 1
Alternate Names
MKNK1
Entrez Gene IDs
8569 (Human)
Gene Symbol
MKNK1
OMIM
606724 (Human)
UniProt
Additional MNK1 Products
Product Documents for MNK1 Antibody (5E2)
Product Specific Notices for MNK1 Antibody (5E2)
This product is produced by and distributed for Abnova, a company based in Taiwan.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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