Moesin Antibody (MSN/492)
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-44579
![Western Blot: Moesin Antibody (MSN/492) [NBP2-44579]](https://resources.bio-techne.com/images/products/Moesin-Antibody-MSN-492-Western-Blot-NBP2-44579-img0009.jpg "Western Blot: Moesin Antibody (MSN/492) [NBP2-44579]")
![Knockout Validated: Moesin Antibody (MSN/492) [NBP2-44579]](https://resources.bio-techne.com/images/products/Moesin-Antibody-MSN-492-Knockout-Validated-NBP2-44579-img0007.jpg "Knockout Validated: Moesin Antibody (MSN/492) [NBP2-44579]")
Conjugate
Catalog #
Forumulation
Catalog #
Key Product Details
Validated by
Knockout/Knockdown
Species Reactivity
Validated:
Human, Rat (Negative)
Cited:
Mouse
Applications
Validated:
Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Knockout Validated, Western Blot
Cited:
Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 kappa Clone # MSN/492
Concentration
0.2 mg/ml
Product Specifications
Immunogen
Recombinant full-length human Moesin protein (Uniprot: P26038 )
Reactivity Notes
Does not react with Rat.
Localization
Cytoplasmic & Cell Surface
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1 kappa
Theoretical MW
78 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Description
200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP2-47916)
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80 C.
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80 C.
Scientific Data Images for Moesin Antibody (MSN/492)
Knockout Validated: Moesin Antibody (MSN/492) [NBP2-44579]
Knockout Validated: Moesin Antibody (MSN/492) [NBP2-44579] - Western blot using lysates from U20S parental cell line and Moesin-1 knockout U20S cell line (KO), collected in RIPA buffer. Nitrocellulose membrane was probed with Mouse Anti-Human/Mouse Moesin Monoclonal Antibody (Catalog # NBP2-44579) at a 1:1000 dilution O/N at 4C, followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody and ECL detection. A specific band was detected for Moesin-1 (as indicated) in the parental U20S cell line, but is not detectable in knockout U20S cell line. The Ponceau stained transfers of each blot are shown to confirm equal protein loading. Image, protocol and testing courtesy of YCharOS Inc. (ycharos.com).![Immunocytochemistry/ Immunofluorescence: Moesin Antibody (MSN/492) [NBP2-44579]](https://resources.bio-techne.com/images/products/Moesin-Antibody-MSN-492-Immunocytochemistry-Immunofluorescence-NBP2-44579-img0005.jpg "Immunocytochemistry/ Immunofluorescence: Moesin Antibody (MSN/492) [NBP2-44579]")
Immunocytochemistry/ Immunofluorescence: Moesin Antibody (MSN/492) [NBP2-44579]
Immunocytochemistry/Immunofluorescence: Moesin Antibody (MSN/492) [NBP2-44579] - Immunofluorescent staining of paraformaldehyde-fixed HeLa cells. followed by goat anti-Mouse IgG-CF488 (Green). The nuclear counterstain is Red Dot (Red)![Immunohistochemistry-Paraffin: Moesin Antibody (MSN/492) [NBP2-44579]](https://resources.bio-techne.com/images/products/Moesin-Antibody-MSN-492-Immunohistochemistry-Paraffin-NBP2-44579-img0001.jpg "Immunohistochemistry-Paraffin: Moesin Antibody (MSN/492) [NBP2-44579]")
Immunohistochemistry-Paraffin: Moesin Antibody (MSN/492) [NBP2-44579]
Immunohistochemistry-Paraffin: Moesin Antibody (MSN/492) [NBP2-44579] - Human Melanoma stained with Moesin Monoclonal Antibody (MSN/492)![Immunohistochemistry-Paraffin: Moesin Antibody (MSN/492) [NBP2-44579]](https://resources.bio-techne.com/images/products/Moesin-Antibody-MSN-492-Immunohistochemistry-Paraffin-NBP2-44579-img0003.jpg "Immunohistochemistry-Paraffin: Moesin Antibody (MSN/492) [NBP2-44579]")
![Flow Cytometry: Moesin Antibody (MSN/492) [NBP2-44579]](https://resources.bio-techne.com/images/products/Moesin-Antibody-MSN-492-Flow-Cytometry-NBP2-44579-img0004.jpg "Flow Cytometry: Moesin Antibody (MSN/492) [NBP2-44579]")
![Western Blot: Moesin Antibody (MSN/492) [NBP2-44579]](https://resources.bio-techne.com/images/products/Moesin-Antibody-MSN-492-Western-Blot-NBP2-44579-img0006.jpg "Western Blot: Moesin Antibody (MSN/492) [NBP2-44579]")
![Immunohistochemistry-Paraffin: Moesin Antibody (MSN/492) [NBP2-44579]](https://resources.bio-techne.com/images/products/Moesin-Antibody-MSN-492-Immunohistochemistry-Paraffin-NBP2-44579-img0002.jpg "Immunohistochemistry-Paraffin: Moesin Antibody (MSN/492) [NBP2-44579]")
![Knockout Validated: Moesin Antibody (MSN/492) [NBP2-44579]](https://resources.bio-techne.com/images/products/Moesin-Antibody-MSN-492-Knockout-Validated-NBP2-44579-img0008.jpg "Knockout Validated: Moesin Antibody (MSN/492) [NBP2-44579]")
Applications for Moesin Antibody (MSN/492)
Application
Recommended Usage
Flow Cytometry
1-2 ug/million cells
Immunocytochemistry/ Immunofluorescence
1-2 ug/ml
Immunohistochemistry-Paraffin
1-2 ug/ml
Western Blot
1-2 ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.
Optimal dilution for a specific application should be determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
10 mM PBS with 0.05% BSA
Preservative
0.05% Sodium Azide
Concentration
0.2 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C.
Background: Moesin
Alternate Names
Membrane-organizing extension spike protein, moesin
Gene Symbol
MSN
UniProt
Additional Moesin Products
Product Documents for Moesin Antibody (MSN/492)
Product Specific Notices for Moesin Antibody (MSN/492)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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