Monoamine Oxidase B Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP1-32426
Key Product Details
Validated by
Orthogonal Validation
Species Reactivity
Validated:
Human, Mouse
Predicted:
Bovine (90%), Canine (94%), Porcine (90%). Backed by our 100% Guarantee.
Applications
ELISA, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Sandwich ELISA, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
Recombinant protein encompassing a sequence within the center region of human Monoamine Oxidase B. The exact sequence is proprietary.
Reactivity Notes
Immunogen displays the following percentage of sequence identity for non-tested species: Rat (86%), Guinea Pig (86%).
Localization
Mitochondrion outer membrane
Marker
Mitochondrial Outer Membrane Marker
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
59 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for Monoamine Oxidase B Antibody
Western Blot: Monoamine Oxidase B Antibody [NBP1-32426]
Western Blot: Monoamine Oxidase B Antibody [NBP1-32426] - HepG2 and mitochondria extracts (30 ug) were separated by SDS-PAGE, and the membrane was blotted with Monoamine Oxidase B antibody [N2C3] diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.Immunocytochemistry/ Immunofluorescence: Monoamine Oxidase B Antibody [NBP1-32426]
Immunocytochemistry/Immunofluorescence: Monoamine Oxidase B Antibody [NBP1-32426] - HepG2 cells were fixed in ice-cold MeOH for 5 min. Green: Monoamine Oxidase B stained by Monoamine Oxidase B antibody [N2C3] diluted at 1:500. Blue: Hoechst 33342 staining.Immunohistochemistry-Paraffin: Monoamine Oxidase B Antibody [NBP1-32426]
Immunohistochemistry-Paraffin: Monoamine Oxidase B Antibody [NBP1-32426] - Mouse liver. Monoamine Oxidase B stained by Monoamine Oxidase B antibody [N2C3] diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 minApplications for Monoamine Oxidase B Antibody
Application
Recommended Usage
ELISA
1:1000-1:10000
Immunocytochemistry/ Immunofluorescence
1:100-1:1000
Immunohistochemistry
1:100-1:1000
Immunohistochemistry-Paraffin
1:100-1:1000
Sandwich ELISA
Assay dependent
Western Blot
1:500-1:3000
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Formulation
PBS (pH 7), 20% Glycerol
Preservative
0.025% Proclin 300
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Background: Monoamine Oxidase B
Long Name
Amine oxidase [flavin-containing] B
Alternate Names
EC 1.4.3.21, EC 1.4.3.4, MAO-B, MAOB
Gene Symbol
MAOB
Additional Monoamine Oxidase B Products
Product Documents for Monoamine Oxidase B Antibody
Product Specific Notices for Monoamine Oxidase B Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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