Monoamine Oxidase B Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP1-59569
Key Product Details
Validated by
Biological Validation
Species Reactivity
Validated:
Human, Mouse
Cited:
Mouse
Applications
Validated:
Immunohistochemistry, Immunohistochemistry-Paraffin, Simple Western, Western Blot
Cited:
Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
0.5 mg/ml
Product Specifications
Immunogen
Synthetic peptides corresponding to MAOB(monoamine oxidase B) The peptide sequence was selected from the N terminal of MAOB. Peptide sequence RDRVGGRTYTLRNQKVKYVDLGGSYVGPTQNRILRLAKELGLETYKVNEV. The peptide sequence for this immunogen was taken from within the described region.
Reactivity Notes
Mouse reactivity reported in scientific literature (PMID: 26841904).
Marker
Mitochondrial Outer Membrane Marker
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
59 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Description
The addition of 50% glycerol is optional for those storing this antibody at -20C and not aliquoting smaller units. However, please note that glycerol may interrupt some downstream antibody applications and should be added with caution.
Scientific Data Images for Monoamine Oxidase B Antibody
Western Blot: Monoamine Oxidase B Antibody [NBP1-59569]
Western Blot: Monoamine Oxidase B Antibody [NBP1-59569] - Mice were pretreated with 5ECdsAP1 aptamer before amphetamine application; tissue samples collected (n = 4 per group) from the VTA at 90 (60 + 30 min) and 180 (60 + 30 + 90 min) minutes after amphetamine. Protein was obtained for Western blot quantitation of MAO-A level using Actin (lower molecular fragments) as a reference shown in lanes 2-4 (90 min samples) or lane 5-6 (180 min). Total protein (10 ug) was used for all lanes except lanes 7 & 9. The blot was stripped and used for MAO-B. Lane 1: molecular size marker of 10 KD ladder; lane 2: 5ECdsAP1 (4 nmol/kg, icv, 1/2 dose); lanes 3 & 5: saline (2 ul, icv); lanes 4 & 6: 5ECdsAP1 (8 nmol/kg, icv); lanes 7, 8, 9 are controls of naive mice with no aptamer or amphetamine (5, 10 and 20 ug protein). Image collected and cropped by CiteAb from the following publication (https://www.jbiomedsci.com/content/23/1/21), licensed under a CC-BY license.Immunohistochemistry-Paraffin: Monoamine Oxidase B Antibody [NBP1-59569]
Immunohistochemistry-Paraffin: Monoamine Oxidase B Antibody [NBP1-59569] - Human Liver tissue.Western Blot: Monoamine Oxidase B Antibody [NBP1-59569]
Western Blot: Monoamine Oxidase B Antibody [NBP1-59569] - Human heart.Applications for Monoamine Oxidase B Antibody
Application
Recommended Usage
Immunohistochemistry
1:10-1:500
Immunohistochemistry-Paraffin
1:10-1:500
Western Blot
1.0 ug/ml
Application Notes
Monoamine Oxidase B Antibody validated for Simple Western from a verified customer review.
Reviewed Applications
Read 1 review rated 3 using NBP1-59569 in the following applications:
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS, 2% Sucrose
Preservative
0.09% Sodium Azide
Concentration
0.5 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: Monoamine Oxidase B
Long Name
Amine oxidase [flavin-containing] B
Alternate Names
EC 1.4.3.21, EC 1.4.3.4, MAO-B, MAOB
Entrez Gene IDs
4129 (Human)
Gene Symbol
MAOB
UniProt
Additional Monoamine Oxidase B Products
Product Documents for Monoamine Oxidase B Antibody
Product Specific Notices for Monoamine Oxidase B Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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