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Key Product Details

Species Reactivity

Mouse, Porcine

Applications

ELISA, Immunocytochemistry

Label

FITC (Excitation = 495 nm, Emission = 519 nm)

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 74-22-15

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

Fresh dd miniature swine thymocytes

Specificity

Porcine SWC3a

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Scientific Data Images for Monocyte/Granulocyte Antibody (74-22-15) [FITC]

Flow Cytometry: Monocyte/Granulocyte Antibody (74-22-15) [FITC] [NBP1-28242] - Porcine peripheral blood lymphocytes, monocytes, and granulocytes were stained with Mouse Anti-Porcine Monocyte/Granulocyte-FITC and Mouse Anti-Porcine CD3 -epsilon- PE.
Flow Cytometry: Monocyte/Granulocyte Antibody (74-22-15) [FITC] [NBP1-28242] - 1 ug/10^6 cells Fresh heparinized blood from a healthy adult pig was stained with mouse anti-pig Monocyte/Granulocyte-FITC. Red blood cells were lyzed with ammonium chloride and the cells were then fixed in 1% paraformaldehyde. Granulocytes were gated and analyzed on a flow cytometer
Flow Cytometry: Monocyte/Granulocyte Antibody (74-22-15) [FITC] [NBP1-28242] - Porcine peripheral blood granulocytes were stained with Mouse Anti-Porcine Monocyte/Granulocyte-PE (NBP1-28244).

Applications for Monocyte/Granulocyte Antibody (74-22-15) [FITC]

Application
Recommended Usage

ELISA

Optimal dilutions of this antibody should be experimentally determined.

Immunocytochemistry

Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

PBS

Preservative

0.1% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: Monocyte/Granulocyte

MAb 74-22-15 reacts with >90% of pig monocytes and granulocytes and <5% of peripheral blood lymphocytes. This clone was clustered as anti-SWC3a at the First International Swine CD Workshop. (1-2)

Alternate Names

3-10C, AMCF-I, C-X-C motif chemokine 8, CXCL8SCYB8, Emoctakin, GCP-1TSG-1, interleukin 8, K60, LECT, MDNCFb-ENAP, member 8, MONAPGCP1, NAP-1NAP1, Neutrophil-activating protein 1, Protein 3-10C, T cell chemotactic factor, T-cell chemotactic factor

Additional Monocyte/Granulocyte Products

Product Documents for Monocyte/Granulocyte Antibody (74-22-15) [FITC]

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Monocyte/Granulocyte Antibody (74-22-15) [FITC]

Protect conjugated forms from light.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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