Mouse ADAM15 Ectodomain Antibody
R&D Systems, part of Bio-Techne | Catalog # AF945
Key Product Details
Species Reactivity
Validated:
Mouse
Cited:
Human, Mouse
Applications
Validated:
Immunohistochemistry, Immunoprecipitation, Western Blot
Cited:
Immunohistochemistry-Frozen, Immunoprecipitation, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Product Specifications
Immunogen
S. frugiperda insect ovarian cell line Sf 21-derived recombinant mouse ADAM15
Arg18-Ser694
Accession # O88839
Arg18-Ser694
Accession # O88839
Specificity
Detects mouse ADAM15 Ectodomain in direct ELISAs and Western blots. In Western blots (under reducing and non-reducing conditions), no cross-reactivity with the ectodomains of recombinant mouse (rm) ADAM9, rmADAM10, recombinant human (rh) ADAM8, rhADAM15, and rhADAM17 is observed.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Applications for Mouse ADAM15 Ectodomain Antibody
Application
Recommended Usage
Immunohistochemistry
5-15 µg/mL
Sample: Perfusion fixed frozen sections of mouse brain (cerebellum)
Sample: Perfusion fixed frozen sections of mouse brain (cerebellum)
Immunoprecipitation
25 µg/mL
Sample: Conditioned cell culture medium spiked with Recombinant Mouse ADAM15, see our available Western blot detection antibodies
Sample: Conditioned cell culture medium spiked with Recombinant Mouse ADAM15, see our available Western blot detection antibodies
Western Blot
0.1 µg/mL
Sample: Recombinant Mouse ADAM15
Sample: Recombinant Mouse ADAM15
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: ADAM15
ADAM15, also known as MDC15 or metargidin, is a widely expressed metalloproteinase. It is a type I membrane protein that may be involved in cell adhesion and shedding of cell surface proteins.
Long Name
A Disintegrin and Metalloprotease-like Domain 15
Alternate Names
MDC15, Metargidin
Gene Symbol
ADAM15
UniProt
Additional ADAM15 Products
Product Documents for Mouse ADAM15 Ectodomain Antibody
Product Specific Notices for Mouse ADAM15 Ectodomain Antibody
For research use only
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