Mouse Aminoacylase/ACY1 Antibody

Name: Mouse Aminoacylase/ACY1 Antibody
Brand: R&D Systems
SKU: MAB2700

R&D Systems, part of Bio-Techne | Catalog # MAB2700

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Product Specifications
Applications
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Species Reactivity

Mouse

Applications

Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Rat IgG_2B Clone # 475730

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Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse Aminoacylase/ACY1
Met1-Ser408
Accession # NP_079647

Specificity

Detects human Aminoacylase/ACY1 in direct ELISAs and Western blots. In Western blots, no cross-reactivity with recombinant human ACY1 is observed.

Clonality

Monoclonal

Host

Rat

Isotype

IgG_2B

Applications for Mouse Aminoacylase/ACY1 Antibody

Application

Recommended Usage

Immunoprecipitation

25 µg/mL
Sample: Cell lysates spiked with Recombinant Mouse Aminoacylase/ACY1 (Catalog # 2700-ZN), see our available Western blot detection antibodies

Western Blot

1 µg/mL
Sample: Recombinant Mouse Aminoacylase/ACY1 (Catalog # 2700-ZN)

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Aminoacylase/ACY1

Aminoacylase 1 (ACY1) is an intracellular soluble homodimeric zinc-binding enzyme. It is involved in the breakdown of acetylated amino acids generated during protein degradation by catalyzing the formation of free aliphatic amino acids from N-acetylated precursors. This enzyme is evolutionarily conserved between mouse and human. Mutations in the human ACY1 gene might cause inborn metabolic disorders. The amino acid sequence of mouse ACY1 is 93% and 85% identical to that of rat and human/chimpanzee.

Alternate Names

ACY1

Entrez Gene IDs

95 (Human); 109652 (Mouse)

Gene Symbol