Mouse Aminoacylase/ACY1 Antibody
R&D Systems, part of Bio-Techne | Catalog # AF2700
Key Product Details
Species Reactivity
Validated:
Mouse
Cited:
Mouse
Applications
Validated:
Immunoprecipitation, Western Blot
Cited:
Immunocytochemistry
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Product Specifications
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse Aminoacylase/ACY1
Met1-Ser408
Accession # NP_079647
Met1-Ser408
Accession # NP_079647
Specificity
Detects mouse Aminoacylase/ACY1 in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 50% cross-reactivity with recombinant human Aminoacylase/ACY1 is observed.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Applications for Mouse Aminoacylase/ACY1 Antibody
Application
Recommended Usage
Immunoprecipitation
25 µg/mL
Sample: Conditioned cell culture medium spiked with Recombinant Mouse Aminoacylase/ACY1 (Catalog # 2700-ZN), see our available Western blot detection antibodies
Sample: Conditioned cell culture medium spiked with Recombinant Mouse Aminoacylase/ACY1 (Catalog # 2700-ZN), see our available Western blot detection antibodies
Western Blot
0.1 µg/mL
Sample: Recombinant Mouse Aminoacylase/ACY1 (Catalog # 2700-ZN)
Sample: Recombinant Mouse Aminoacylase/ACY1 (Catalog # 2700-ZN)
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Aminoacylase/ACY1
The mouse ACY1 gene encodes aminoacylase, a member of the M20 family of metalloproteases (1). ACY1 plays a general role in the cytosolic breakdown of acetylated amino acids generated during protein degradation. It also interacts with sphingosine kinase type 1, which is involved in promoting cell growth and inhibiting apoptosis of tumor cells (2). The full-length protein is expressed and the purified enzyme is active in the assay.
References
- Lindner, H.A. et al. (2003) J. Biol. Chem. 278:44496.
- Maceyka, M. et al. (2004) FEBS Lett. 568:30.
Alternate Names
ACY1
Gene Symbol
ACY1
UniProt
Additional Aminoacylase/ACY1 Products
Product Documents for Mouse Aminoacylase/ACY1 Antibody
Product Specific Notices for Mouse Aminoacylase/ACY1 Antibody
For research use only
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