Mouse Autoimmune Regulator/AIRE Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB6184
Conjugate
Catalog #
Key Product Details
Species Reactivity
Validated:
Mouse
Applications
CyTOF-ready, Intracellular Staining by Flow Cytometry, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Rat IgG2A Clone # 609930
Product Summary for Mouse Autoimmune Regulator/AIRE Antibody
Immunogen
E. coli-derived recombinant mouse AIRE
Ser476-Ser552 (predicted)
Accession # Q9Z0E3
Ser476-Ser552 (predicted)
Accession # Q9Z0E3
Specificity
Detects mouse AIRE in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human AIRE is observed.
Clonality
Monoclonal
Host
Rat
Isotype
IgG2A
Scientific Data Images for Mouse Autoimmune Regulator/AIRE Antibody
Detection of Mouse Autoimmune Regulator/AIRE by Western Blot.
Western blot shows lysates of mouse splenocytes and mouse thymus tissue. PVDF Membrane was probed with 1 µg/mL of Mouse Autoimmune Regulator/AIRE Monoclonal Antibody (Catalog # MAB6184) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). Specific bands were detected for Autoimmune Regulator/AIRE at approximately 70 and 48 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Detection of Autoimmune Regulator/AIRE in Mouse splenocytes by Flow Cytometry.
Mouse splenocytes were stained with Rat Anti-Mouse Autoimmune Regulator/AIRE Monoclonal Antibody (Catalog # MAB6184, filled histogram) or isotype control antibody (Catalog # MAB006, open histogram), followed by Phycoerythrin-conjugated Anti-Rat IgG Secondary Antibody (Catalog # F0105B). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with saponin.Applications for Mouse Autoimmune Regulator/AIRE Antibody
Application
Recommended Usage
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
Intracellular Staining by Flow Cytometry
2.5 µg/106 cells
Sample:
Sample:
Mouse splenocytes fixed with paraformaldehyde and permeabilized with saponin.
Western Blot
1 µg/mL
Sample: Mouse splenocytes and mouse thymus tissue
Sample: Mouse splenocytes and mouse thymus tissue
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Autoimmune Regulator/AIRE
AutoImmune REgulator (AIRE) is an approximately 60 kDa nuclear and cytosolic protein that is required for the development of T cell tolerance. It regulates the expression of self-antigens by thymic epithelial cells, and mutations in AIRE are causative of the autoimmune disorder, APECED. AIRE regulates gene transcription through interactions with DNA, histone H3, and the nuclear matrix. It contains one HSD domain (aa 1-105), a nuclear localization sequence (aa 113-133), one SAND domain (aa 181-280), and two PHD zinc finger domains (aa 299-340 and aa 434-475). Alternate splicing of human AIRE generates isoforms that lack the HSR and SAND domains and/or the second PHD domain. Within aa 476-552, human AIRE shares 65% and 63% aa sequence identity with mouse and rat AIRE, respectively.
Alternate Names
AIRE, APECED, APS1, APSI, PGA1
Gene Symbol
AIRE
UniProt
Additional Autoimmune Regulator/AIRE Products
Product Documents for Mouse Autoimmune Regulator/AIRE Antibody
Product Specific Notices for Mouse Autoimmune Regulator/AIRE Antibody
For research use only
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