Mouse c-Rel Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB2699
Key Product Details
Species Reactivity
Validated:
Mouse
Cited:
Mouse
Applications
Validated:
Western Blot
Cited:
Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Rat IgG2A Clone # 290512
Product Specifications
Immunogen
E. coli-derived recombinant mouse c-Rel
Met1-Ile588
Accession # P15307
Met1-Ile588
Accession # P15307
Specificity
Detects endogenous mouse c-Rel in Western blots.
Clonality
Monoclonal
Host
Rat
Isotype
IgG2A
Scientific Data Images for Mouse c-Rel Antibody
Detection of Mouse c-Rel by Western Blot.
Western blot shows lysates of DA3 mouse myeloma cell line, A20 mouse B cell lymphoma cell line, and CH-1 mouse B cell lymphoma cell line. PVDF membrane was probed with 0.5 µg/mL of Mouse c-Rel Monoclonal Antibody (Catalog # MAB2699) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for c-Rel at approximately 80 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.Applications for Mouse c-Rel Antibody
Application
Recommended Usage
Western Blot
0.5 µg/mL
Sample: DA3 mouse myeloma cell line, A20 mouse B cell lymphoma cell line, and CH-1 mouse B cell lymphoma cell line
Sample: DA3 mouse myeloma cell line, A20 mouse B cell lymphoma cell line, and CH-1 mouse B cell lymphoma cell line
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: c-Rel
c-Rel is a member of the NF kappaB/Rel family of transcription factors. NF kappaB/Rel dimers containing c-Rel regulate expression of genes involved in lymphoid cell development, proliferation, and survival.
Long Name
Cellular Repressor of E1A-stimulated Genes
Alternate Names
cRel, REL
Gene Symbol
REL
UniProt
Additional c-Rel Products
Product Documents for Mouse c-Rel Antibody
Product Specific Notices for Mouse c-Rel Antibody
For research use only
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