Mouse Carboxypeptidase B1/CPB1 Antibody
R&D Systems, part of Bio-Techne | Catalog # AF2898
Key Product Details
Species Reactivity
Validated:
Mouse
Cited:
Mouse
Applications
Validated:
Immunoprecipitation, Western Blot
Cited:
ELISA Development, Immunohistochemistry, Immunoprecipitation, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Product Specifications
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse Carboxypeptidase B1/CPB1
Ser16-Tyr415
Accession # NP_083982
Ser16-Tyr415
Accession # NP_083982
Specificity
Detects mouse Carboxypeptidase B1/CPB1 in direct ELISAs and Western blots. In Western blots, approximately 10% cross-reactivity with recombinant human (rh) CPB1 is observed and less than 1% cross-reactivity with rhCPA1 and recombinant mouse CPA4 is observed.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Applications for Mouse Carboxypeptidase B1/CPB1 Antibody
Application
Recommended Usage
Immunoprecipitation
25 µg/mL
Sample: Conditioned cell culture medium spiked with Recombinant Mouse Carboxypeptidase B1/CPB1 (Catalog # 2898-ZN), see our available Western blot detection antibodies
Sample: Conditioned cell culture medium spiked with Recombinant Mouse Carboxypeptidase B1/CPB1 (Catalog # 2898-ZN), see our available Western blot detection antibodies
Western Blot
0.1 µg/mL
Sample: Recombinant Mouse Carboxypeptidase B1/CPB1 (Catalog # 2898-ZN)
Sample: Recombinant Mouse Carboxypeptidase B1/CPB1 (Catalog # 2898-ZN)
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Reviewed Applications
Read 1 review rated 4 using AF2898 in the following applications:
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Carboxypeptidase B1/CPB1
Carboxypeptidase B1, encoded by the CPB1 gene, specifically cleaves the C-terminal Arg and Lys residues with a preference for Arg (1). The deduced amino acid sequence of mouse CPB1 consists of a signal peptide (residues 1 to 15), a pro region (residue 16 to 108), and a mature chain (residues 109 to 415). The purified rmCPB1 corresponds to the pro form, which can be activated by trypsin, the only pancreatic protease capable of generating active enzyme from the zymogen in vitro (1).
References
- Aviles, F.X. and J. Vendrell (2004) in Handbook of Proteolytic Enzymes (ed. Barrett, et al.) pp. 831, Academic Press, San Diego.
Alternate Names
CPB1, PASP, PCPB
Gene Symbol
CPB1
UniProt
Additional Carboxypeptidase B1/CPB1 Products
Product Documents for Mouse Carboxypeptidase B1/CPB1 Antibody
Product Specific Notices for Mouse Carboxypeptidase B1/CPB1 Antibody
For research use only
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