Mouse Caspase-11/Caspase-4 Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB8648
Recombinant Monoclonal Antibody.
Key Product Details
Validated by
Biological Validation
Species Reactivity
Mouse
Applications
Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # 1172C
Product Specifications
Immunogen
HEK293 human embryonic kidney cell line transfected with mouse Caspase-11/Caspase-4
Accession # P70343
Accession # P70343
Specificity
Detects mouse Caspase-11/Caspase-4 in Western blots.
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for Mouse Caspase-11/Caspase-4 Antibody
Detection of Mouse Caspase-11/Caspase-4 by Western Blot.
Western blot shows lysates of mouse splenocytes untreated (-) or treated (+) with LPS. PVDF membrane was probed with 1 µg/mL of Rabbit Anti-Mouse Caspase-11/Caspase-4 Monoclonal Antibody (Catalog # MAB8648) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). Specific bands were detected for Caspase-11/Caspase-4 at approximately 36, 44, and 46 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.Applications for Mouse Caspase-11/Caspase-4 Antibody
Application
Recommended Usage
Western Blot
1 µg/mL
Sample: Mouse splenocytes treated with LPS
Sample: Mouse splenocytes treated with LPS
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Reviewed Applications
Read 1 review rated 4 using MAB8648 in the following applications:
Formulation, Preparation, and Storage
Purification
Protein A or G purified from cell culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Caspase-11/Caspase-4
Alternate Names
CASP11, Caspl, Ich-3
Gene Symbol
CASP4
UniProt
Additional Caspase-11/Caspase-4 Products
Product Documents for Mouse Caspase-11/Caspase-4 Antibody
Product Specific Notices for Mouse Caspase-11/Caspase-4 Antibody
For research use only
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