Mouse CCL11/Eotaxin Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB420
Key Product Details
Species Reactivity
Validated:
Mouse
Cited:
Mouse
Applications
Validated:
Immunohistochemistry, Neutralization, Western Blot
Cited:
Neutralization, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Rat IgG2A Clone # 42285
Product Specifications
Immunogen
E. coli-derived recombinant mouse CCL11/Eotaxin
His24-Pro97
Accession # P48298
His24-Pro97
Accession # P48298
Specificity
Detects mouse CCL11/Eotaxin in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 10% cross-reactivity with recombinant human (rh) CCL11/Eotaxin, recombinant mouse CCL7, and rhCCL21 is observed.
Clonality
Monoclonal
Host
Rat
Isotype
IgG2A
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Scientific Data Images for Mouse CCL11/Eotaxin Antibody
Chemotaxis Induced by CCL11/Eotaxin and Neutralization by Mouse CCL11/Eotaxin Antibody.
Recombinant Mouse CCL11/ Eotaxin (Catalog # 420-ME) chemoattracts the Y3 rat myeloid cell line transfected with human CCR3 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by MTT staining. Chemotaxis elicited by Recombinant Mouse CCL11/ Eotaxin (0.1 µg/mL) is neutralized (green line) by increasing concentrations of Rat Anti-Mouse CCL11/Eotaxin Monoclonal Antibody (Catalog # MAB420). The ND50 is typically 3-30 µg/mL.
CCL11/Eotaxin in Rat Intestine.
CCL11/Eotaxin was detected in perfusion fixed frozen sections of rat intestine using Rat Anti-Mouse CCL11/Eotaxin Monoclonal Antibody (Catalog # MAB420) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Rat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS017) and counterstained with hematoxylin (blue). Specific staining was localized to fibroblasts in submucosae. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.Applications for Mouse CCL11/Eotaxin Antibody
Application
Recommended Usage
Immunohistochemistry
8-25 µg/mL
Sample: Perfusion fixed frozen sections of rat intestine
Sample: Perfusion fixed frozen sections of rat intestine
Western Blot
1 µg/mL
Sample: Recombinant Mouse CCL11/Eotaxin (Catalog # 420-ME)
Sample: Recombinant Mouse CCL11/Eotaxin (Catalog # 420-ME)
Neutralization
Measured by its ability to neutralize CCL11/Eotaxin-induced chemotaxis in the Y3 rat myeloid cell line transfected with human CCR3. The Neutralization Dose (ND50) is typically 3-30 µg/mL in the presence of 0.1 µg/mL Recombinant Mouse CCL11/Eotaxin.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: CCL11/Eotaxin
CCL11 is a potent eosinophil chemoattractant that was originally purified from bronchoalveolar lavage fluid of guinea pigs sensitized by aerosol challenge with ovalbumin. Mouse CCL11 cDNA encodes a 97 amino acid (aa) precursor protein from which the amino-terminal 23 aa are cleaved to generate the 74 aa mature mouse CCL11. At the protein sequence level, mature mouse CCL11 is approximately 60% identical to mature human and guinea pig CCL11. In addition, mouse CCL11 also shows high aa sequence identity to members of the MCP family. Mouse CCL11 is chemotactic for eosinophils, but not mononuclear cells or neutrophils. CCL11 mRNA is expressed in a variety of tissues. The expression of CCL11 mRNA is induced in cultured endothelial cells in response to IFN-gamma. In addition, CCL11 mRNA is also induced in response to the transplantation of IL-4-secreting tumor cells. The CC chemokine receptor 3 (CCR3) has been identified as a specific human CCL11 receptor.
References
- Rothenberg, M.E. et al. (1995) Proc. Natl. Acad. Sci. USA 92:8960.
- Kitaura, M. et al. (1996) J. Biol. Chem 271:7725.
- Garcia-Zepeda, E.A. et al. (1996) Nature Medicine 2:449.
- Ponath, P.D. et al. (1996) J. Clin. Invest. 97:604.
Alternate Names
Eotaxin
Gene Symbol
CCL11
UniProt
Additional CCL11/Eotaxin Products
Product Documents for Mouse CCL11/Eotaxin Antibody
Product Specific Notices for Mouse CCL11/Eotaxin Antibody
For research use only
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