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Mouse CD2 Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB2828

R&D Systems, part of Bio-Techne

Key Product Details

Species Reactivity

Mouse

Applications

CyTOF-ready, Flow Cytometry, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Rat IgG2A Clone # 377830

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse CD2
Arg23-Ser203
Accession # P08920

Specificity

Detects mouse CD2 in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant human CD2 is observed.

Clonality

Monoclonal

Host

Rat

Isotype

IgG2A

Applications for Mouse CD2 Antibody

Application
Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

2.5 µg/106 cells
Sample: Mouse CD3+ splenocytes

Western Blot

1 µg/mL
Sample: Recombinant Mouse CD2
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Reconstitution Buffer Available:
Size / Price
Qty
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Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CD2

CD2 is a type I glycoprotein belonging to the Ig superfamily and is expressed on T, NK, B, and some antigen presenting cells. Human CD2 binds human CD58 on antigen-presenting cells to induce co-stimulating signals in T cells.

Alternate Names

CD2, LFA-2, T11

Entrez Gene IDs

914 (Human); 12481 (Mouse); 102120798 (Cynomolgus Monkey)

Gene Symbol

CD2

UniProt

Additional CD2 Products

Product Documents for Mouse CD2 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Mouse CD2 Antibody

For research use only

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