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Mouse Common  gamma Chain/IL-2 R gamma Antibody

R&D Systems, part of Bio-Techne | Catalog # AF784

R&D Systems, part of Bio-Techne
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AF784
AF784-SP

Key Product Details

Species Reactivity

Validated:

Mouse

Cited:

Mouse

Applications

Validated:

Neutralization, Western Blot

Cited:

ELISA Development (Capture), Neutralization, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Product Specifications

Immunogen

S. frugiperda insect ovarian cell line Sf 21-derived recombinant mouse Common gamma Chain/IL‑2 R gamma
Ser25-Ala263
Accession # Q3UPA9

Specificity

Detects mouse Common gamma Chain/IL‑2 R gamma in direct ELISAs and Western blots. In Western blots, approximately 5% cross-reactivity with recombinant human (rh) IL-2 R gamma is observed and less than 1% cross-reactivity with rhIL-2 R alpha, recombinant mouse IL-2 R beta, and rhIL-15 R is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Endotoxin Level

<0.10 EU per 1 μg of the antibody by the LAL method.

Scientific Data Images for Mouse Common  gamma Chain/IL-2 R gamma Antibody

Cell Proliferation Induced by IL‑15 and Neutralization by Mouse Common  gamma Chain/ IL‑2 R gamma Antibody.

Cell Proliferation Induced by IL‑15 and Neutralization by Mouse Common gamma Chain/ IL‑2 R gamma Antibody.

Recombinant Human IL-15 (Catalog # 247-IL) stimulates proliferation in the CTLL-2 mouse cytotoxic T cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human IL-15 (0.8 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Mouse Common gamma Chain/IL-2 R gamma Antigen Affinity-purified Poly-clonal Antibody (Catalog # AF784). The ND50 is typically 6-24 µg/mL.

Applications for Mouse Common  gamma Chain/IL-2 R gamma Antibody

Application
Recommended Usage

Western Blot

0.1 µg/mL
Sample: Recombinant Mouse Common  gamma Chain/IL-2 R gamma Fc Chimera (Catalog # 784-MR)

Neutralization

Measured by its ability to neutralize IL-15-induced proliferation in the CTLL-2 mouse cytotoxic T cell line. The Neutralization Dose (ND50) is typically 6-24 µg/mL in the presence of 0.8 ng/mL Recombinant Human IL-15.

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Reconstitution Buffer Available:
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Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Common gamma Chain/IL-2 R gamma

The gamma chain of the high affinity functional mouse IL-2 receptor complex belongs to the hematopoietin receptor family. Mouse Common gamma Chain is a 369 amino acid residue protein consisting of a 22 residue signal sequence, a 241 residue extracellular domain, a 21 residue transmembrane domain and an 85 residue cytoplasmic domain. Although Common gamma Chain by itself does not bind IL-2 with any appreciable affinity, it is required for IL-2 receptor signaling. Besides IL-2, the gamma chain has been shown to be a component of the functional receptor complexes for IL-4, IL-7, IL-9 and IL-15. IL-2 R gamma is also designated the common gamma chain ( gammac) (1, 2). The site of molecular defects in human X-linked SCID (severe combined immunodeficiency) has been mapped to the human common gamma gene.

References

  1. Minami, Y. et al. (1993) Annu. Rev. Immunol. 11:245.
  2. Noguchi, M. et al. (1993) Science 262:1877.

Long Name

IL-2 Receptor gamma

Alternate Names

CD132, IL-2 R gamma, IL2R gamma, IL2RG

Entrez Gene IDs

3561 (Human); 16186 (Mouse)

Gene Symbol

IL2RG

UniProt

Additional Common gamma Chain/IL-2 R gamma Products

Product Documents

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Mouse Common  gamma Chain/IL-2 R gamma Antibody

For research use only

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