Mouse CRELD1 Antibody
R&D Systems, part of Bio-Techne | Catalog # AF4116
Key Product Details
Species Reactivity
Mouse
Applications
Immunohistochemistry, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Product Specifications
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse CRELD1
Gln30-Glu362
Accession # Q91XD7
Gln30-Glu362
Accession # Q91XD7
Specificity
Detects mouse CRELD1 in direct ELISAs and Western blots. In Western blots, approximately 15% cross-reactivity with recombinant human CRELD1 is observed and less than 5% cross-reactivity with recombinant mouse CRELD2 is observed.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for Mouse CRELD1 Antibody
CRELD1 in Mouse Embryo.
CRELD1 was detected in immersion fixed frozen sections of mouse embryo (E13.5) using Goat Anti-Mouse CRELD1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4116) at 10 µg/mL overnight at 4 °C. Tissue was stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to the developing heart and is shown at both low (lower panel) and high magnification (upper panel). View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections.Applications for Mouse CRELD1 Antibody
Application
Recommended Usage
Immunohistochemistry
5-15 µg/mL
Sample: Immersion fixed frozen sections of embyronic mouse heart (E13.5)
Sample: Immersion fixed frozen sections of embyronic mouse heart (E13.5)
Western Blot
0.1 µg/mL
Sample: Recombinant Mouse CRELD1
Sample: Recombinant Mouse CRELD1
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: CRELD1
CRELD1 (cysteine-rich with EGF-like domain protein 1) is an integral membrane protein that functions as a cell adhesion molecule. It is synthesized as a 420 amino acid (aa) precursor with an N-terminal signal peptide, a large extracellular domain (ECD) with two EGF-like repeats that flank two consecutive furin-like repeats, two‑transmembrane domains separated by one cytoplasmic residue, and a short extracellular C-terminal domain. CRELD1 null-mouse fails to develop a normal heart and missense mutations in human CRELD1 have also been associated with atrioventricular septal defects. The N-terminal ECD of mouse CRELD1 shares 97% and 92% aa sequence identity with the ECD of rat and human CRELD1, respectively.
Long Name
Cysteine-rich Protein With EGF-like Domains 1
Alternate Names
AVSD2, CIRRIN
Gene Symbol
CRELD1
UniProt
Additional CRELD1 Products
Product Documents for Mouse CRELD1 Antibody
Product Specific Notices for Mouse CRELD1 Antibody
For research use only
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