Mouse Cryptic Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB18401
Conjugate
Catalog #
Key Product Details
Species Reactivity
Mouse
Applications
CyTOF-ready, Flow Cytometry, Immunocytochemistry
Label
Unconjugated
Antibody Source
Monoclonal Rat IgG2B Clone # 221428
Product Specifications
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse Cryptic
Tyr36-Gly177
Accession # P97766
Tyr36-Gly177
Accession # P97766
Specificity
Detects mouse Cryptic in direct ELISAs and Western blots. In Western blots, 100% cross-reactivity with recombinant human Cryptic is observed.
Clonality
Monoclonal
Host
Rat
Isotype
IgG2B
Scientific Data Images for Mouse Cryptic Antibody
Cryptic in D3 Mouse Cell Line.
Cryptic was detected in immersion fixed D3 mouse embryonic stem cell line using Rat Anti-Mouse Cryptic Monoclonal Antibody (Catalog # MAB18401) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rat IgG Secondary Antibody (red; Catalog # NL013) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm and nuclei. View our protocol for Fluorescent ICC Staining of Stem Cells on Coverslips.Applications for Mouse Cryptic Antibody
Application
Recommended Usage
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
Flow Cytometry
0.25 µg/106 cells
Sample: D3 mouse embryonic stem cell line
Sample: D3 mouse embryonic stem cell line
Immunocytochemistry
8-25 µg/mL
Sample: Immersion fixed D3 mouse embryonic stem cell line
Sample: Immersion fixed D3 mouse embryonic stem cell line
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Cryptic
Cryptic, also known as CFC-1, is a member of the Epidermal Growth Factor-Cripto/FRL-1/Cryptic (EGF-CFC) family of signaling proteins. It is expressed during mesoderm differentation and is necessary for determining left-right asymmetry. Cryptic functions as a co-receptor that regulates signaling by some TGF-beta superfamily members.
Alternate Names
CFC-1, CFC1
Gene Symbol
CFC1
UniProt
Additional Cryptic Products
Product Documents for Mouse Cryptic Antibody
Product Specific Notices for Mouse Cryptic Antibody
For research use only
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