Mouse Cystatin E/M Antibody

Name: Mouse Cystatin E/M Antibody
Brand: R&D Systems
SKU: MAB1284
Rating: 5 (2 reviews)

R&D Systems, part of Bio-Techne | Catalog # MAB1284

(2)

Product Datasheet / COA / SDS

100% Guarantee

Product Specifications
Applications
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Species Reactivity

Mouse

Applications

Immunoprecipitation, Neutralization, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Rat IgG_2B Clone # 216820

View all Cystatin E/M Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse Cystatin E/M
Glu29-Val149
Accession # NP_082899

Specificity

Detects mouse Cystatin E/M in direct ELISAs and Western blots. In Western blots, no cross-reactivity with recombinant human (rh) Cystatin B, S, E/M, SA, S/N, recombinant mouse (rm) Cystatin A, C, D, rhFetuin A, B, rmHPRG, or rhKininogen is observed.

Clonality

Monoclonal

Host

Rat

Isotype

IgG_2B

Applications for Mouse Cystatin E/M Antibody

Application

Recommended Usage

Immunoprecipitation

25 µg/mL
Sample: Conditioned cell culture medium spiked with Recombinant Mouse Cystatin E/M (Catalog # 1284-PI), see our available Western blot detection antibodies

Western Blot

1 µg/mL
Sample: Recombinant Mouse Cystatin E/M (Catalog # 1284-PI)

Neutralization

Measured by its ability to neutralize Recombinant Mouse Cystatin E/M (0.64 µg/mL, Catalog # 1284-PI) inhibition of Papain (0.1 µg/mL) cleavage of the fluorogenic peptide substrate Z-FR-AMC (100 µM, Catalog # ES009). The Neutralization Dose (ND₅₀) is typically 7 µg/mL.

Please Note: Optimal dilutions of this antibody should be experimentally determined.

Reviewed Applications

Read 2 reviews rated 5 using MAB1284 in the following applications:

Western Blot (2 Reviews)

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Cystatin E/M

Cystatin E/M is a member of family 2 of the cystatin superfamily (1 ‑ 6). It is required for viability and for correct formation of cornified layers in the epidermis and hair follicles, as ichq mice with a null mutation in the cystatin E/M gene have defects in epidermal cornification and die between 5 and 12 days of age (7). Cystatin E/M expression and function may not be limited to cutaneous epithelia. For example, it is found in rat brain and is induced during neuronal cell differentiation (8). In addition to being a cysteine protease inhibitor, the human cystatin E/M is also a substrate for transglutaminases (9).

References

Carninci, P. and Y. Hayashizaki (1999) Meth. Enzymol. 303:19.
Carninci, P. et al. (2000) Genome Res. 10:1617.
Shibata, K. et al. (2000) Genome Res. 10:1757.
Kawai, J. et al. (2001) Nature 409:685.
Sotiropoulou, G. et al. (1997) J. Biol. Chem. 272:903.
Ni, J. et al. (1997) J. Biol. Chem. 272:10853.
Zeeuwen, P.L. et al. (2002) Hum. Mol. Genet. 11:2867.
Hong, J. et al. (2002) J. Neurochem. 81:922.
Zeeuwen, P.L. et al. (2001) J. Invest. Dermatol. 116:693.

Alternate Names

CST6

Entrez Gene IDs

1474 (Human); 73720 (Mouse)

Gene Symbol