Mouse Cystatin/Stefin Homolog 2 Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB1516
Key Product Details
Species Reactivity
Mouse
Applications
Immunoprecipitation, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Rat IgG2B Clone # 237305
Product Specifications
Immunogen
E. coli-derived recombinant mouse Cystatin/Stefin Homolog 2
Met2-Phe97
Accession # NP_084009
Met2-Phe97
Accession # NP_084009
Specificity
Detects mouse Cystatin/Stefin Homolog 2 in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant human (rh) Cystatin D, S, SA, SN, recombinant mouse Cystain B, C, E/M, rhFetuin A, rhFetuin B, rhHPRG, or rhKininostatin is observed.
Clonality
Monoclonal
Host
Rat
Isotype
IgG2B
Applications for Mouse Cystatin/Stefin Homolog 2 Antibody
Application
Recommended Usage
Immunoprecipitation
25 µg/mL
Sample: Conditioned cell culture medium spiked with Recombinant Mouse Cystatin H2, see our available Western blot detection antibodies
Sample: Conditioned cell culture medium spiked with Recombinant Mouse Cystatin H2, see our available Western blot detection antibodies
Western Blot
1 µg/mL
Sample: Recombinant Mouse Cystatin/Stefin Homolog 2
Sample: Recombinant Mouse Cystatin/Stefin Homolog 2
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Cystatin/Stefin Homolog 2
RIKEN cDNA clone 2010005H15 (Nucleotide Accession # AK008122) predicted an intracellular protein, which was similar to members of the cystatin superfamily (1‑4). The protein is designated here as mouse Cystatin/Stefin Homolog 2 (Cystatin H2) because the deduced amino acid sequence showed the highest identity (75% over the entire length) to that of mouse cystatin/stefin homolog (SwissProt Accession # P56567).
References
- Carninci, P. and Hayashizaki, Y. (1999) Methods. Enzymol. 303:19.
- Carninci, P. et al. (2000) Genome Res. 10:1617.
- Shibata, K. et al. (2000) Genome Res. 10:1757.
- Kawai, J. et al. (2001) Nature 409:685.
Alternate Names
Cystatin H2
Entrez Gene IDs
76770 (Mouse)
Gene Symbol
CSTA2
UniProt
Additional Cystatin/Stefin Homolog 2 Products
Product Documents for Mouse Cystatin/Stefin Homolog 2 Antibody
Product Specific Notices for Mouse Cystatin/Stefin Homolog 2 Antibody
For research use only
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