Mouse Endoglycan/PODXL2 Antibody
R&D Systems, part of Bio-Techne | Catalog # AF3534
Key Product Details
Species Reactivity
Validated:
Mouse
Cited:
Mouse, Porcine
Applications
Validated:
CyTOF-ready, Flow Cytometry, Immunohistochemistry, Western Blot
Cited:
Immunohistochemistry
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Product Specifications
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse Endoglycan/PODXL2
Gly30-Gly498
Accession # Q8CAE9
Gly30-Gly498
Accession # Q8CAE9
Specificity
Detects mouse Endoglycan/PODXL2 in direct ELISAs and Western blots.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for Mouse Endoglycan/PODXL2 Antibody
Endoglycan/PODXL2 in Mouse Testis.
Endoglycan/PODXL2 was detected in perfusion fixed frozen sections of mouse testis using 1.7 µg/mL Goat Anti-Mouse Endoglycan/PODXL2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3534) overnight at 4 °C. Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counter-stained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.Applications for Mouse Endoglycan/PODXL2 Antibody
Application
Recommended Usage
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
Flow Cytometry
2.5 µg/106 cells
Sample: bEnd.3 mouse endothelioma cell line
Sample: bEnd.3 mouse endothelioma cell line
Immunohistochemistry
5-15 µg/mL
Sample: Perfusion fixed frozen sections of mouse testis
Sample: Perfusion fixed frozen sections of mouse testis
Western Blot
0.1 µg/mL
Sample: Recombinant Mouse Endoglycan/PODXL2
Sample: Recombinant Mouse Endoglycan/PODXL2
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Endoglycan/PODXL2
Alternate Names
PODLX2, PODXL2
Gene Symbol
PODXL2
UniProt
Additional Endoglycan/PODXL2 Products
Product Documents for Mouse Endoglycan/PODXL2 Antibody
Product Specific Notices for Mouse Endoglycan/PODXL2 Antibody
For research use only
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