Mouse EPCR Biotinylated Antibody

R&D Systems, part of Bio-Techne | Catalog # BAF2749

R&D Systems, part of Bio-Techne
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BAF2749
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AF2749
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Key Product Details

Species Reactivity

Mouse

Applications

Flow Cytometry, Immunocytochemistry, Western Blot

Label

Biotin

Antibody Source

Polyclonal Goat IgG

View all EPCR Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse EPCR
Ala17-Ser214
Accession # Q64695

Specificity

Detects mouse EPCR in Western blots. In Western blots, approximately 10% cross-reactivity with recombinant human EPCR is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Mouse EPCR Biotinylated Antibody

Detection of EPCR antibody in bEnd.3 Mouse Cell Line antibody by Flow Cytometry.

Detection of EPCR in bEnd.3 Mouse Cell Line by Flow Cytometry.

bEnd.3 mouse endothelioma cell line was stained with Goat Anti-Mouse EPCR Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF2749, filled histogram) or control antibody (Catalog # BAF108, open histogram), followed by Streptavidin-Allophycocyanin (Catalog # F0050).

Applications for Mouse EPCR Biotinylated Antibody

Application
Recommended Usage

Flow Cytometry

2.5 µg/106 cells
Sample: bEnd.3 mouse endothelioma cell line

Immunocytochemistry

5-15 µg/mL
Sample: Immersion fixed bEND.3 mouse endothelioma cell line

Western Blot

0.1 µg/mL
Sample: Recombinant Mouse EPCR

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Reconstitution Buffer Available:
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Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: EPCR

Protein C is a vitamin K-dependent serine protease that plays a major role in blood coagulation. Binding of Protein C to EPCR (CD201) leads to the proteolytic activation of PAR1 (protease-activated receptor 1) on endothelial cells and subsequent up-regulation of Protein C-induced genes. EPCR is a type I transmembrane glycoprotein in the CD1/MHC family. It is expressed most strongly in the endothelial cells of arteries and veins in heart and lung. Membrane bound EPCR is released by metalloproteolytic cleavage to generate the soluble receptor. The extracellular domain of human and mouse EPCR shares approximately 61% amino acid sequence homology.

Long Name

Endothelial Protein C Receptor

Alternate Names

CCD41, CD201, PROCR

Entrez Gene IDs

10544 (Human); 19124 (Mouse)

Gene Symbol

PROCR

UniProt

Q64695

Additional EPCR Products

Product Documents for Mouse EPCR Biotinylated Antibody

Product Datasheets

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Product Specific Notices for Mouse EPCR Biotinylated Antibody

For research use only

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