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Mouse ErbB2/Her2 Alexa Fluor® 594-conjugated Antibody

R&D Systems, part of Bio-Techne | Catalog # FAB6744T

R&D Systems, part of Bio-Techne

Key Product Details

Species Reactivity

Validated:

Mouse

Cited:

Mouse

Applications

Validated:

Flow Cytometry

Cited:

Flow Cytometry

Label

Alexa Fluor 594 (Excitation = 590 nm, Emission = 617 nm)

Antibody Source

Monoclonal Rat IgG1 Clone # 666521

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse ErbB2/Her2
Thr23-Thr653
Accession # P70424

Specificity

Detects mouse ErbB2/Her2 in direct ELISAs. In direct ELISAs, less than 5% cross-reactivity with recombinant human ErbB2, recombinant mouse (rm) ErbB3, rmErbB4, and rmEGF R is observed.

Clonality

Monoclonal

Host

Rat

Isotype

IgG1

Scientific Data Images for Mouse ErbB2/Her2 Alexa Fluor® 594-conjugated Antibody

Detection of ErbB2/Her2 in NIH-3T3 Mouse Cell Line by Flow Cytometry.

NIH-3T3 mouse embryonic fibroblast cell line was stained with Rat Anti-Mouse ErbB2/Her2 Alexa Fluor® 594-conjugated Monoclonal Antibody (Catalog # FAB6744T, filled histogram) or isotype control antibody (IC005T, open histogram). Staining was performed using our Staining Membrane-associated Proteins protocol.
Detection of ErbB2/Her2 antibody in 3T3-L1 Mouse Cell Line antibody by Flow Cytometry.

Detection of ErbB2/Her2 in 3T3‑L1 Mouse Cell Line by Flow Cytometry.

3T3-L1 mouse embryonic fibroblast adipose-like cell line was stained with Rat Anti-Mouse ErbB2/Her2 Alexa Fluor® 594-conjugated Monoclonal Antibody (Catalog # FAB6744T, filled histogram) or isotype control antibody (Catalog # IC005T, open histogram). View our protocol for Staining Membrane-associated Proteins.

Applications for Mouse ErbB2/Her2 Alexa Fluor® 594-conjugated Antibody

Application
Recommended Usage

Flow Cytometry

0.25 - 1.0 µg/106 cells
Sample: 3T3‑L1 mouse embryonic fibroblast adipose-like cell line and NIH-3T3 mouse fibroblast cell line

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: ErbB2/Her2

ErbB2, also known as CD340, Neu, and Her2 (Human Epidermal Growth Factor Receptor 2), is a 180-185 kDa type I transmembrane glycoprotein that is a member of the Class I family of tyrosine kinase receptors. ErbB2 is widely expressed on and in epithelial cells, and plays a role in development, cancer, communication at the neuromuscular junction, and regulation of cell growth and differentiation. The mouse ErbB2 extracellular domain (ECD, amino acids 23-653) contains two "L" domains and two cystine-rich domains.  Within the ECD, it shares 85% and 94% amino acid sequence identity with human and rat ErbB2 ECD, respectively. The protease ADAM10 releases a 95-110 kDa soluble fragment of ErbB2 from the cell surface. ErbB2 is also known to undergo alternative splicing, and to appear in the nucleus as part of a transcriptional regulatory complex.  ErbB2 has no identified ligands, but heterodimerizes with ErbB1 (EGF R), ErbB3, or ErbB4 to form higher affinity signaling complexes. It also appears to form a complex with Plexin B1, c-met, and Mucin-4.  Within those complexes, non-EGF ligands are likely involved.  The ErbB2/ErbB3 heterodimer is expressed in the majority of breast, skin, ovary and gastrointestinal tumors and transduces a highly mitogenic signal in response to neuregulin 1 (NRG1; heuregulin 1) or NRG2.

Long Name

Receptor Tyrosine Protein Kinase ErbB2

Alternate Names

CD340, HER2, Neu Oncogene, NGL, TKR1

Entrez Gene IDs

2064 (Human); 13866 (Mouse); 24337 (Rat); 102146608 (Cynomolgus Monkey)

Gene Symbol

ERBB2

UniProt

Additional ErbB2/Her2 Products

Product Documents

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Mouse ErbB2/Her2 Alexa Fluor® 594-conjugated Antibody


This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.

For research use only

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